Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body

ABSTRACT: Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body

PROVIDER: PRJNA905411 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22408672_1.fastq.gz	Fastqsanger.gz
	SRR22408672_2.fastq.gz	Fastqsanger.gz
	SRR22408673_1.fastq.gz	Fastqsanger.gz
	SRR22408673_2.fastq.gz	Fastqsanger.gz
	SRR22408674_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 58

Similar Datasets

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (RNA-seq in a derived population)

Project description:We used RNA-seq in a derived European Drosophila melanogaster population from Germany (MU) to examine coding gene expression variation in the larval fat body during the late wandering third instar stage.

2023-04-04 | GSE218763 | GEO

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (pooled RNA-seq in a derived and an ancestral population)

Project description:We used RNA-seq to examine coding and non-coding gene expression variation in the larval fat body of an ancestral African and a derived European Drosophila melanogaster population across three developmental stages spanning ten hours of larval development.

2023-04-04 | GSE220321 | GEO

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (RNA-seq in a derived population)

Project description:Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (RNA-seq in a derived population)

| PRJNA905415 | ENA

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body.

Project description:Gene expression variation is pervasive across all levels of organismal organization, including development. Few studies, however, have examined variation in developmental transcriptional dynamics among populations, or how it contributes to phenotypic divergence. Indeed, the evolution of gene expression dynamics when both the evolutionary and temporal timescale are comparatively short remains relatively uncharacterized. Here, we examined coding and non-coding gene expression in the fat body of an ancestral African and a derived European Drosophila melanogaster population across three developmental stages spanning ten hours of larval development. Between populations, expression divergence was largely stage-specific. We detected higher expression variation during the late wandering stage, which may be a general feature of this stage. During this stage, we also detected higher and more extensive lncRNA expression in Europe, suggesting that lncRNA expression may be more important in derived populations. Interestingly, the temporal breadth of protein-coding and lncRNA expression became more restricted in the derived population. Taken together with the signatures of potential local adaptation that we detected at the sequence level in 9-25% of candidate genes (those showing evidence of expression divergence between populations), this finding suggests that gene expression becomes more developmental stage-specific during adaptation to new environments. We further used RNAi to identify several candidate genes that likely contribute to known phenotypic divergence between these populations. Our results shed light on the evolution and dynamics of expression variation over short developmental and evolutionary timescales, and how this variation contributes to population and phenotypic divergence.

| S-EPMC10166500 | biostudies-literature

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (pooled RNA-seq in a derived and an ancestral population)

Project description:Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (pooled RNA-seq in a derived and an ancestral population)

| PRJNA909634 | ENA

The mode of expression divergence in Drosophila fat body is infection-specific

Project description:Transcription is controlled by the interactions of cis-acting DNA elements with diffusible trans-acting factors. Changes in cis or trans factors can drive expression divergence within and between species, and the relative prevalence of each can reveal the evolutionary path to variation. Previous work delineating the mode of expression divergence in animals has largely used whole body expression measurements in a single condition. Since cis-acting elements often drive expression in a subset of cell types or conditions, these measurements may not capture the complete contribution of cis-acting changes. Here, we quantify the mode of expression divergence in the Drosophila fat body, the primary immune organ, in several conditions. We performed allele-specific expression analysis using two geographically distinct lines of D. melanogaster and their F1 hybrids. We performed separate infections with Gram-negative S. marcescens or Gram-positive E. faecalis bacteria, which trigger the two primary signaling pathways in the Drosophila innate immune response. The mode of expression divergence strongly depends on the condition, with trans-acting effects dominating in response to Gram-positive infection and cis-acting effects dominating in Gram-negative and pre-infection conditions. Expression divergence in several receptor proteins may underlie the infection-specific trans effects. Before infection, when the fat body has a metabolic role, there are many compensatory effects, changes in cis and trans that counteract each other to maintain expression levels. This work demonstrates that within a single tissue, the mode of expression divergence varies between conditions and suggests that these differences reflect the diverse evolutionary histories of host-pathogen interactions.

2021-03-23 | GSE155033 | GEO

Expression profile of third instar larval fat body and midgut tissues

2010-11-02 | GSE25025 | GEO

Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-04-04 | GSE218762 | GEO

Expression profile of third instar larval fat body and midgut tissues

Project description:We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

2010-11-02 | E-GEOD-25025 | biostudies-arrayexpress