Transcriptomic profiling reveals differential cellular response of copper oxide nanoparticles and polystyrene nanoplastics in perfused human placenta
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PROVIDER: PRJNA911283 | ENA |
REPOSITORIES: ENA
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Project description:Concerns about the potential risks to human health due nanoparticulate pollution have been emerging. However, the risks to sensitive populations, such as pregnant individuals and their unborn children are poorly characterised. With increasing evidence of environmental particles passing the placenta, their potential adverse effects of on pregnancy and fetal development need to be assessed. Here, we investigated the impact of copper oxide (CuO) and polystyrene (PS) nanoparticle exposure on gene expression in ex vivo perfused human placental tissue.
2023-10-01 | GSE220756 | GEO
Project description:Nanoplastics are produced by breakdown of plastics in environmental contamination or commercial use for cosmetics or daily expenses. Emerging evidence indicate that ingested nanoplastics with a size smaller than 100 nm have the potential to reach the brain and induces neurotoxicity. However, the potential toxicity of nanoplastics on brain are limited because of difficulties in synthesize of nanoplastics. In present study, we synthesized the fluorescent polystyrene nanoplastics (PSNPs) and examined the toxicity of PSNPs in brain in vivo and in vitro analyses by comparison to IR-813 fluorophore. Synthesized PSNPs were characterized by fluorescence imaging system, scanning electron microscopy, and Fourier-transform infrared spectroscopy. PSNPs were detected in adult mice brain by oral ingestion. In addition, a series of behavioral analyses showed that oral ingestion of PSNPs induced memory impairments. Among brain cells, PSNPs were predominantly internalized in microglia, and uptake of PSNPs induced microglial activation. In addition, the conditioned medium derived from microglia exposed to PSNPs repressed hippocampal neuronal activity. Furthermore, transcriptome analysis showed that PSNPs changed gene expressions in microglia, elevation of neuroinflammation in contrast to suppression of neurotrophic factors. These results indicated that predominant uptake of PSNPs in microglia induced elevation of neuroninflammatory responses whereas suppression of neurotrophic factors that may contribute to the cognitive impairment. Our findings indicate the toxic mechanism and potential detrimental effect of nanoplastic in brain and suggest a potential risk of cognitive impairment by exposure to nanoplastics.
2023-09-24 | GSE243612 | GEO
Project description:Micro- and nanoplastics (MNPLs) have emerged as pervasive environmental pollutants, raising growing concerns about their potential health risks. Their documented presence in the human bloodstream highlights the need to understand their interactions with endothelial cells and their role in cardiovascular diseases such as atherosclerosis. However, the full implications of these interactions remain largely unknown. To address this, we investigated the impact of three sizes (30, 50, and 100 nm) of polystyrene carboxylated nanoplastics (PS-C-NPLs) on human umbilical vein endothelial cells (HUVECs) at 100 µg/mL. By integrating in vitro assays with bioinformatic transcriptomic analyses, we employed a multimodal approach to comprehensively investigate the effects of PS-C-NPLs on endothelial cells. In vitro assessments included nanoplastics (NPLs) internalisation (flow cytometry, confocal microscopy, TEM), morphological and internal complexity changes (flow cytometry), genotoxicity (comet assay), and functional alterations, such as cholesterol biosynthesis (Filipin III staining), migration (wound healing assay), angiogenesis, and inflammation (IL-6 ELISA). Additionally, RNA sequencing provided transcriptomic insights into the cellular response to PS-C-NPLs, complementing the in vitro findings and revealing molecular pathways underlying the observed effects. All three PS-C-NPL sizes were rapidly internalised by HUVECs within 20 minutes, inducing significant alterations in cell morphology, internal complexity, and function. PS-C-NPLs caused genotoxic damage and disrupted cholesterol metabolism, migration, angiogenesis, and inflammatory responses. Notably, some effects exhibited a size-dependent trend, with similarities emerging between carboxylated polystyrene (PS-C) 50 and 100 nm NPLs, while the smallest 30 nm NPL showed slightly distinct responses. Transcriptomic analyses reinforced these findings, revealing shared pathways across all three PS-C-NPLs —linked to cholesterol metabolism, endothelial-to-mesenchymal transition, DNA damage, and inflammation — alongside size-specific molecular signatures. This study is the first to comprehensively link transcriptomic changes to size-dependent functional alterations in endothelial cells induced by PS-C-NPLs. Our findings demonstrate that PS-C-NPLs significantly impair endothelial cell function and integrity in a size-dependent manner, underscoring their potential cardiovascular risks.
2026-04-22 | GSE324731 | GEO
Project description:Male C57BL/6 mice were assigned to three groups (n=6 per group): ISO, ISO+100 nm polystyrene nanoplastics (PS), and ISO+1 µm PS. Hearts were collected at the study endpoint and profiled by bulk mRNA-seq to identify transcriptional signatures associated with PS size in the setting of ISO-induced myocardial injury. Libraries were prepared and sequenced in two batches (Batch 1 by Novogene; Batch 2 by Majorbio Bio-Pharm Technology).
2026-03-28 | GSE322523 | GEO
Project description:We used LC-MS to detect the protein expression in liver, the main organ of orally injected polystyrene nanoplastics accumulated, so that we can analyze the function change of liver upon polystyrene nanoplastics exposure.
2025-11-07 | PXD070447 |
Project description:Nanoplastics represent an emerging class of global environmental contaminants; however, their potential to interfere with human developmental processes remains largely unexplored. This study investigated the impact of exposure to polystyrene nanoplastics (PS-NP) on the de novo formation, differentiation, and function of human liver organoids derived from induced pluripotent stem cells (hiPSCs), a sophisticated model of early organogenesis.
2025-12-01 | GSE298927 | GEO
Project description:In this study, the proteins forming the protein corona on PS and PVC were investigated after 5 min, 1 h, 6 h, or 24 h incubation of the nanoplastics with human plasma. TMT-based untargeted proteomics were applied to identify the proteins forming the protein corona.
2024-10-25 | PXD032162 | Pride
Project description:Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
| PRJNA1436950 | ENA
Project description:<p>The uptake and accumulation of nanoplastics by plants have raised particular concerns. However, molecular mechanisms underlying nanoplastic phytotoxicity are still vague and insufficient. Thus, its potential environmental risks and biosafety are receiving increased attention. In this study, cucumber plants were exposed to polystyrene nanoplastics (PSNPs) under different concentration. The results demonstrated that phenotypic parameters (total photosynthetic pigment content, relative membrane permeability, total phenolic content, and lipid peroxidation) were not significantly affected. However, metabolomic and transcriptomic analysis revealed that both doses of PSNPs induced the expression regulation of different key genes, exhibiting considerable distinction on metabolic responses in cucumber. The high dose of PSNPs induced stronger alteration in cucumber leaves than low dose of PSNPs. Low dose exposure significantly affected four metabolic pathways involved in the carbohydrate metabolism. High dose exposure significantly affected five metabolic pathways involved in the carbohydrate metabolism and four metabolic pathways involved in the amino acid metabolism. These findings shed new light on the phytotoxic mechanism and environmental implication behind the interaction of nanoplastics and plants, and advance our understanding of the potential adverse effect induced by the presence of nanoplastics in agricultural systems.</p>
2024-09-27 | MTBLS3923 | MetaboLights
Project description:Nanoplastics (NPs), plastic particles smaller than 1 μm, have gained increasing attention due to their potential health effects. Experimental studies in animals have shown that NPs can pass biological barriers, reach the female reproductive tract, and enter the ovary. Their presence in the ovary might have profound consequences for reproductive endpoints, such as oocyte development. This study aimed to investigate the uptake and associated effects of polystyrene (PS) NPs on oocyte and embryo development using a bovine in vitro fertilization (IVF) model. Bovine cumulus-oocyte complexes (COCs) were exposed to pristine PS-NPs (50 nm or 200 nm) at different doses during in vitro maturation (23 hours), followed by IVF and embryo culture. RNA sequencing was performed to investigate mechanisms involved in possible effects on oocyte maturation. Confocal microscopy confirmed the internalization of 50 nm and 200 nm PS-NPs into cumulus cells, however, only 50 nm particles were able to transfer into oocytes. Additionally, exposure to 50 nm PS-NPs at 3 μg/mL significantly delayed oocyte nuclear maturation and early embryo development. At this dose, RNA sequencing did not identify differentially expressed genes in the COCs, however, gene set enrichment analyses did indicate effects on mitochondrial processes. Nevertheless, subsequent analysis on mitochondrial activity remained unaffected.
2026-02-06 | GSE299785 | GEO