Project description:Priestia endophytica FH5, which was isolated from healthy tomato rhizosphere soil, had biological activity against a variety of plant diseases, including R. solani. We isolated the chemicals generated by strain FH5 to better understand the interaction between strain FH5 and R. solani. A transcriptome study of strain FH5 with and without R. solani exposure was also performed. In response to the fungal pathogen R. solani, strain FH5 changed genes linked to amino acid transport, carbohydrate transport, energy generation and conversion, and inorganic ion transport and metabolism, according to our findings.
Project description:Polyhydroxyalkanoates (PHAs) production using cellulosic biomass is a promising way for sustainable manufacturing of bioplastics. The famous bacterium, Priestia megaterium, is an ideal choice because it can utilize glucose and xylose for PHA synthesis. In the present study, we engineered the genome of P. megaterium by CRISPR-Cas9 system to enhance cellobiose utilization and PHA production. The genes encoding for β-glucosidases (Bgls) from different microbes were introduced into the P. megaterium to improve the cellobiose utilization.
