Project description:Elucidation of the molecular envenomation strategy of the cone snail Conus geographus through transcriptome sequencing of its venom duct
Project description:The venom of cone snails is highly variable both between and within species, as well as spatially along the venom duct. However, defferences of defensive and predatory venoms in "hook-and-line" fish hunting clades and their venom duct origins has not been investigated. In this study a combination of proteomics and transcriptomic approaches were used to decode the venom profiles of C. striatus from the Pionoconus clade. The raw data files obtained from the reduced alkylated and digested venom duct sections (distal, central and proximal), injected predatory and defensive induced venoms are submitted here.
Project description:Combined Proteomic and Transcriptomic Interrogation of the Venom Gland of Conus geographus Uncovers Novel Components and Functional Compartmentalization
Project description:This study aimed to describe the proteomic profile of Conus regius venom through proteomic analysis. Venom samples were fractionated using reversed-phase chromatography and analyzed by Q-TOF mass spectrometry. The resulting spectra were analyzed using Peaks Studio V7.0 software and a Conus protein database. A total of 520 peptide and protein sequences were identified, primarily in the 5-10 kDa molecular mass range, with most corresponding to conopeptides.