RNA array data of abdominal adipose tissue post duodenal-jejunal bypass (DJB)
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PROVIDER: PRJNA916219 | ENA |
REPOSITORIES: ENA
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Project description:To analyze the mRNA, lncRNA, and circRNA expression profile in abdominal adipose tissues post DJB surgery, high-fat diet induced typical diabetic mice were underformed sham surgery or DJB surgery. Two weeks post the surgery, mice were enthanized and the abdominal adipose tissues were collected for RNA array analysis. To analyze the ceRNA networks regulated by DJB surgery
2023-12-22 | GSE221792 | GEO
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Project description:Metabolic and bariatric surgery (MBS) can generate a drastic shift of coding and non-coding RNAs expression pattern, which triggers organ function remodeling and may induce type 2 diabetes (T2D) remission. Our previous studies demonstrated that the altered expression profiles of duodenal and jejunal long noncoding RNAs (lncRNAs) after the duodenal-jejunal bypass (DJB), an investigational procedure and research tool of MBS, can improve glycemic control by modulating entero-pancreatic axis and gut-brain axis, respectively. As an indiscerptible part of intestine, the ileal lncRNAs expression signatures after DJB and the critical pathways associated with postoperative correction of impaired metabolism need to be investigated
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Project description:FCHL is a common, complex genetic lipid disorder with a largely unknown aetiology. Altered adipose tissue metabolism has been implicated as contributing to FCHL. We used microarrays to investigate the mRNA profiles of FCHL patients with CHD and non-FCHL patients with CHD in comparison with non-FCHL, non-CHD patients to define a core set of genes that were differentially expressed in FCHL and to provide a reasonable launch-pad for examining the direct contribution of one of this gene set to the development of adipose tissue. Adipose tissue was obtained from the same anatomical site - the subcutaneous, upper abdominal region - of white British males, either at coronary artery bypass surgery (designated as: FCHL-CHD (13 individuals, subdivided on TG response to statins into Group 1 (n=4), Group 2 (n=7) and unclassified (n=2)) and non-FCHL-CHD patients (6 individuals)), or at heart valve replacement: non-FCHL, non-CHD subjects (5 individuals; controls).
2013-10-29 | E-GEOD-51625 | biostudies-arrayexpress
Project description:Comparison of subcutaneous abdominal adipose tissue before and after biliopancreatic diversion with duodenal switch (BPD/DS), and versus subcutaneous abdominal adipose tissue from lean, healthy subjects undergoing hernia repair surgery
2010-06-10 | E-TABM-862 | biostudies-arrayexpress
Project description:Aim: The aim of the study is to identify the regulatory role of intestinal sweet taste receptors (STRs) and glucose transporters (SGLT1, GLUT2) and gut peptide secretion in duodenal-jejunal bypass (DJB)-ameliorated glycemic control in Type 2 diabetes. Materials and Methods: DJB and sham surgeries were performed in streptozotocin-induced diabetic male rats. The blood GLP-1 and GLP-2 levels were evaluated under feeding and fasting conditions. The expression of STRs (T1R2, T1R3), sweet taste signaling effector (Gα-gustducin), SGLT1, and GLUT2 was detected in the intestinal alimentary limb (A limb), biliopancreatic limb (BP limb), and common limb (C limb). The effects of STR inhibition on glucose control were measured with lactisole. Results: Glucose tolerance was improved in DJB-operated rats compared with the sham group, similar to that of normal control rats, without significant differences in food intake and body weight. The plasma GLP-1 levels of DJB rats were increased under diet-fed condition, and GLP-2 levels were increased after fasting. The villus height and crypt depth were significantly increased in the A limb of DJB-operated rats. In addition, GLP-1 expression was restored in enterocytes. The expression of T1R2, Gα-gustducin, and SGLT1 was elevated in the A limb after DJB, while GLUT2 was downregulated in the A, BP, and C limbs. The localization of GLUT2 was normalized in the three intestinal limbs after DJB. However, the beneficial effects of DJB on glucose control were abolished in the presence of lactisole in vivo. Conclusion: DJB ameliorates glycemic control probably by restoring STR-mediated glucose sensing and absorption with the responses of GLP-1 and GLP-2 to carbohydrate.
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Project description:Surgical conversion of Roux-en-Y gastric bypass (RYGB) to one anastomosis duodenal switch with sleeve gastrectomy (SADI-S), can be effective, when there is obesity recidivism, but surgically challenging. This case report video aims to detail the technical modifications that simplifies this conversion, in one stage. This video article demonstrates the conversion of RYGB to SADI-S using a jejunal bridge to facilitating the gastro-gastric reconnection. Surgical conversion was done laparoscopically, firstly removing the fundus, gastric body and the proximal part of the antrum. The gastrojejunal (GJ) anastomosis from the previous RYGB was preserved and the jejunal alimentary limb that follows was transected, 8cm distal to the GJ anastomosis, and anastomosed, at this level, with the antrum. The remaining alimentary limb was removed, until the jejuno-jejuno anastomosis, from the previous RYGB. The interposition of a segment of jejunal alimentary limb between the gastric bypass pouch and the antrum, has shown to be safe and feasible in RYGB conversion to SADI-S, without complications. Not reconnecting the remnant jejunal alimentary limb to the intestinal transit, but removing it, makes the procedure shorter and safer. Graphical abstract Image, graphical abstract
| S-EPMC9808024 | biostudies-literature
Project description:Objective: Adipose tissue plays a key role in obesity related metabolic dysfunction. MicroRNA (miRNA) are gene regulatory molecules involved in inter-cellular and inter-organ communication. We hypothesised that miRNA levels in adipose tissue would change after gastric bypass surgery and that this would provide insights into their role in obesity-induced metabolic dysregulation. Methods: miRNA-profiling (Affymetrix_Gene-Chip_miRNA2.0_Arrays) of omental and subcutaneous adipose (n=15 females) before, and after, gastric bypass surgery. Results: One omental, and thirteen subcutaneous adipose miRNAs were significantly, differentially expressed after gastric bypass, including down-regulation of miR-223-3p and its antisense relative, miR-223-5p, in both adipose tissues. mRNA levels of miR-223-3p targets NLRP3 and GLUT4 were increased and decreased respectively following gastric bypass in both adipose tissues. Significantly more NLRP3 protein was observed in omental adipose after gastric bypass (P=0.02). Significant hypomethlyation of NLRP3 and hypermethylation of miR-223 was observed in both adipose tissues after gastric bypass. In subcutaneous adipose significant correlations were observed between both miR-223-3p and miR-223-5p and glucose, and between NLRP3 mRNA and protein levels and blood lipids. Conclusions: This is the first report detailing genome-wide miRNA-profiling of omental adipose before and after gastric bypass, and further highlights a link between miR-223-3p and the NLRP3 inflammasome in obesity.
2019-12-16 | E-MTAB-8594 | biostudies-arrayexpress
Project description:To explore the potential mechanism of duodeno-jejunal bypass (DJB) in improving insulin resistance in diabetic rats, we suggest that adipose tissue is involved in the regulation. To investigate genetic changes in adipose tissue after duodeno-jejunal bypass, we performed RNA-seq analysis to establish the relationship between growth hormone, adiponectin, and insulin sensitivity by measuring changes in growth hormone pathways, including downstream JAK2 and MAPK pathways, and investigated non-PPAR signaling pathways. This study deepens our understanding of the complex mechanism by which DJB affects insulin resistance. This meticulous exploration fills an important gap in the literature and may pave the way for more targeted and effective diabetes management interventions.
2023-09-22 | GSE243812 | GEO