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Deletion of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero

ABSTRACT: Deletion of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero

PROVIDER: PRJNA917442 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22950100_1.fastq.gz	Fastqsanger.gz
	SRR22950100_2.fastq.gz	Fastqsanger.gz
	SRR22950101_1.fastq.gz	Fastqsanger.gz
	SRR22950101_2.fastq.gz	Fastqsanger.gz
	SRR22950102_1.fastq.gz	Fastqsanger.gz

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Deletion of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero

Project description:The hypothesis that CSF2 plays a role in the preimplantation development of the bovine embryo was tested by evaluating consequences of deletion of CSF2RA (the functional receptor in the embryo) for development of embryos in utero. CRISPR/Cas9 was used to delete exon 5 and intron 5 of CSF2RA on chromosome 3. Control embryos were injected with Cas9 mRNA only. Embryos > 16 cells at day 5 after insemination were transferred to synchronized recipient females in groups of 7 to 24. Embryos were flushed from the uterus two days later. The proportion of recovered embryos that developed to the blastocyst stage was lower for knockout embryos (39%) than for control embryos (63%). RNA sequencing of individual morulae and blastocysts indicated a total of 27 (morula) or 15 (blastocyst) differentially-expressed genes (false discovery rate <0.05). Differentially-expressed genes as well as regulated gene sets identified by gene set enrichment analysis indicated that knockout affected genes playing roles in several functions including cell signaling and glycosylation. It was concluded that signaling through CSF2RA is not obligatory for development of the bovine preimplantation embryo to the blastocyst stage but that CSF2 signaling does enhance the likelihood that the embryo can become a blastocyst and result in specific changes in gene expression.

2023-05-10 | GSE222003 | GEO

Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero.

Project description:The hypothesis that CSF2 plays a role in the preimplantation development of the bovine embryo was tested by evaluating consequences of inactivation of CSF2RA (the functional receptor in the embryo) for development of embryos in utero. CRISPR/Cas9 was used to alter sequences on exon 5 and intron 5 of CSF2RA, Control embryos were injected with Cas9 mRNA only. Embryos > 16 cells at day 5 after insemination were transferred to synchronized recipient females in groups of 7 to 24. Embryos were flushed from the uterus two days later. The proportion of recovered embryos that developed to the blastocyst stage was lower for knockout embryos (39%) than for control embryos (63%). RNA sequencing of individual morulae and blastocysts indicated a total of 27 (morula) or 15 (blastocyst) differentially-expressed genes (false discovery rate <0.05). Gene set enrichment analysis indicated that the knockout affected genes playing roles in several functions including cell signaling and glycosylation. It was concluded that signaling through CSF2RA is not obligatory for development of the bovine preimplantation embryo to the blastocyst stage but that CSF2 signaling does enhance the likelihood that the embryo can become a blastocyst and result in specific changes in gene expression.

| S-EPMC10160533 | biostudies-literature

Preimplantation mouse embryo development MOE430B

Project description:Preimplantation mouse embryo development on Affymetrix MOE430B. Zeng et al (2004) Dev Biol 272:483-496. Keywords: other

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Project description:Preimplantation mouse embryo development study on Affymetrix U74Av2. Zeng et al Dev Biol (2004) 272:483-496. Keywords: other

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Preimplantation mouse embryo development MOE430A

Project description:Preimplantation mouse embryo development study on Affymetrix MOE430A. Zeng et al (2004) Dev Biol 272:483-496. Keywords: other

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Transcription profiling of preimplantation mouse embryo development

Project description:Preimplantation mouse embryo development study on Affymetrix MOE430A. Zeng et al (2004) Dev Biol 272:483-496.

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Analysis of Chinese sheep preimplantation embryo development and block by single-cell DNA methylome sequencing

Project description:The early stages of mammalian embryonic development involve the participation and cooperation of numerous complex processes, including nutritional, genetic, and epigenetic mechanisms. However, in embryos cultured in vitro, a developmental block occurs that affects embryo development and the efficiency of culture. Although the block period is reported to involve the transcriptional repression of maternal genes and transcriptional activation of zygotic genes, how epigenetic factors regulate developmental block is still unclear. In this study, we systematically analyzed whole-genome methylation levels during five stages of sheep oocyte and preimplantation embryo development using SC-WGBS technology. Then, we examined several million CpG sites in individual cells at each evaluated developmental stage to identify the methylation changes that take place during the development of sheep preimplantation embryos. Our results showed that two strong waves of methylation changes occurred, namely, demethylation at the 8- to 16-cell stage and methylation at the 16- to 32-cell stage. Analysis of DNA methylation patterns in different functional regions revealed a stable hypermethylation status in 3'UTRs and gene bodies; however, significant differences were observed in intergenic and promoter regions at different developmental stages. Changes in methylation at different stages of preimplantation embryo development were also compared to investigate the molecular mechanisms involved in sheep embryo development at the methylation level. In conclusion, we report a detailed analysis of the DNA methylation dynamics during the development of sheep preimplantation embryos. Our results provide an explanation for the complex regulatory mechanisms underlying the embryo developmental block based on changes in DNA methylation levels.

2021-12-15 | GSE190746 | GEO

Isoform-resolved transcriptome of the human preimplantation embryo [PacBio]

Project description:Human preimplantation development is a complex process involving extensive remodeling of gene expression. However, the preimplantation embryo transcriptome has only been annotated using short-read sequencing, which fails to capture full-length mRNAs and associated isoform diversity. We present a novel human embryo transcriptome using integrated long- and short-read RNA sequencing data. Our analysis reveals a total of 110,212 novel isoforms transcribed from known genes containing either a novel combination of known splice sites or at least one novel splice site, and 17,964 isoforms transcribed from completely novel genes located either in antisense direction of known genes or in intergenic space.

2023-09-26 | GSE190547 | GEO

Isoform-resolved transcriptome of the human preimplantation embryo [Illumina]

2023-09-26 | GSE190544 | GEO

Isoform-resolved transcriptome of the human preimplantation embryo

Project description:Isoform-resolved transcriptome of the human preimplantation embryo

| PRJNA787537 | ENA