Project description:In this study, we firstly obtained the complete mitochondrial genome DNA sequence of the Ambassis gymnocephalus. Its length is 17,388 bp, containing 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a large noncoding region. The overall length of protein coding genes is 11,448 bp, including A (26.75%), G (15.20%), T (26.44%) and C (31.61%). The A + T content of control region (1555 bp) is 60.45%. The protein-coding gene COI is started by GTG and ended by AGA. From the phylogenetic tree, we find A. gymnocephalus and Oreochromis niloticus has the closest relationship among 21 related species. This work will provide some valuable information for further studies on A. gymnocephalus and the family Ambassidae.
Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.
Project description:Whole genome sequencing of the Arabidopsis thaliana dot5-1 transposon insertion line described in Petricka et al 2008 The Plant Journal 56(2): 251-263.
Project description:The analysis identifies differentially occupied genomic regions of H2Bub1, H3K79me3, and H3K27ac by RNF40 silencing in HCC1806 cells
