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Characterization of the RNA present in L1 ORF1p macromolecular complexes from Mael-/- mouse testes

ABSTRACT: Characterization of the RNA present in L1 ORF1p macromolecular complexes from Mael-/- mouse testes

PROVIDER: PRJNA922048 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23021829.fastq.gz	Fastqsanger.gz
	SRR23021830.fastq.gz	Fastqsanger.gz
	SRR23021831.fastq.gz	Fastqsanger.gz
	SRR23021832.fastq.gz	Fastqsanger.gz
	SRR23021833.fastq.gz	Fastqsanger.gz

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Characterization of the RNA present in L1 ORF1p macromolecular complexes from Mael-/- mouse testes

Project description:piRNA-deficient Maelstrom (Mael) null mice are characterized by a strong upregulation of LINE-1 (L1) retrotransposon in meiotic spermatocytes. This defect turns out in the accumulation of L1 RNA and ORF1p in their cytoplasm and the formation of prominent ribonucleoprotein aggregates. We used 3-months-old Mael-/- male mice to characterize the RNA present in those ORF1p aggregates. To favor the isolation of complexed versus free ORF1p protein, we first fractionated Mael-/- testis extracts (that we refer to as TOTAL) by sucrose gradient ultracentrifugation, in the presence of EDTA. We then pooled the sucrose fractions where ORF1p macromolecular complexes sediment (fractions 5-8) and used this pool as the INPUT for an anti-ORF1p co-immunoprecipitation (IP) followed by RNA-seq.

2023-05-25 | GSE222416 | GEO

Characterization of L1 ORF1p macromolecular complexes from Mael-/- mouse testes

Project description:piRNA-deficient Maelstrom (Mael) null mice are characterized by a strong upregulation of retrotransposon LINE-1 (L1) in meiotic spermatocytes. This defect turns out in the accumulation of L1 RNA and ORF1p in their cytoplasm and the formation of prominent ribonucleoprotein aggregates. We used 3-months-old Mael-/- male mice to characterize the protein composition of those ORF1p aggregates.

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