Project description:Proteomic data of 8 groups of samples from four strains of Phaeodactylum Tricornutum, which were respectively control group and grazing stress group. Four strains of P. tricornutum (CCAP 1052/1A, CCAP 1052/1B, CCAP 1055/3, CCAP 1055/7) were provided by Génomique, Environnementale et Evolutive Section 3 CNRS UMR8197, Institut de Biologie de l'ENS (IBENS).
Project description:Loss of DNA methylation is traditionally associated with transcriptional up-regulation of transposable elements. Here we describe for the first time expression changes upon loss of DNA methylation in diatoms using Phaeodactylum tricornutum as a model species. The loss of DNA methylation was obtained by generation of DNMT5 KOs, a divergent DNMT with SNF-like domains. Expression analysis confirm the loss of DNA methylation is associated with de-repression of transposable elements in Phaeodactylum tricornutum with indirect effects on protein coding genes.