Project description:In this study, it is noticeable that 32 tea-specific miRNAs were confirmed on the base of genome survey, using deep sequencing and microarray hybridization, and many miRNAs might associate with secondary metabolites synthesis.
Project description:We performed the genomic sequencing, assembly and annotation of T. pullulans strain CRUB 1754 (Perito Moreno glacier, Argentina), a gene survey of Carbohydrate-active enzymes (CAZymes); and analyzed its secretome by Liquid-Chromatography coupled to tandem Mass Spectrometry (LC-MS/MS) after growth in glucose (GLU) or starch (STA) as main carbon sources.
Project description:The overall objective of the study was to survey the cellular and gene expression heterogeneity of human muscle tissue by single-cell RNA-sequencing.
Project description:We report the survey of two repressive epigenetic marks in hand-dissected mature Arabidopsis embryos. DNA from approximately 2500 embryos was extracted for each samples. DNA was treated with bisulfite and fractionated for whole genome bisulfite sequencing to reveal methylated cytosines. Chromatin was fractionnated and immuno-precipited with either anti-H3K9me2 or anti-H3 to test for histone methylation. Both profiles were compared in different mutant backgrounds to survey how small RNA influence reprogramming in the embryo.
Project description:We used small-RNA sequencing to carry out the first comprehensive survey of miRNAs expressed in SAR1A-knockout K562 cells without or with HU treatment and assessed their potential functions related to HbF induction. our findings suggest a profile of dysregulated miRNAs associated with SAR1A downregulation which may influence HbF expression directly or indirectly targeting the transcription factors linked to gamma-globin gene regulation.
