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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

ABSTRACT: The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

PROVIDER: PRJNA929268 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23266850.fastq.gz	Fastqsanger.gz
	SRR23266851.fastq.gz	Fastqsanger.gz
	SRR23266852.fastq.gz	Fastqsanger.gz
	SRR23266853.fastq.gz	Fastqsanger.gz
	SRR23266854.fastq.gz	Fastqsanger.gz

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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not completely understood. Here, we have studied antigen-specific assay systems, which revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors such as Amphiregulin (Areg). Tissue remodeling could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN- and TNF. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with an effector program promoting Areg, TNF and IFNg expression, and a TCR-linkage analysis demonstrated a strong clonal relation between tissue and blood PD1+TIGIT+TOX+ CD8 T cells with tissue remodeling abilities. This regenerative program was also present in anti-cancer associated CD8 populations including chimeric antigen receptor (CAR) CD8 T cells. Once activated, the tissue regenerative program of human CD8 T cells enhanced tumor spheroid, as well as primary liver/bile duct organoid growth. Our findings may help to understand the conflicting CD8 biology in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies for solid tumors.

2023-11-20 | GSE223988 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

Project description:The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

2023-11-20 | GSE223987 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not understood. Here, antigen-specific assay systems revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors. Tissue remodeling by CD8 T cells could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN-Ã¯ÂÂ§ and TNF. The regenerative program also enhanced tumor spheroid and primary organoid growth. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with a strong TCR clonal relation to blood PD1+TIGIT+ CD8 T cells with tissue remodeling abilities. Our findings may help to understand CD8 in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies.

| EGAS00001006960 | EGA

HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes

2003-03-10 | E-GEOD-336 | biostudies-arrayexpress

Dhx9 controls effector CD8 T cell differetiation program

Project description:Dhx9 controls effector CD8 T cell differetiation program

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HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

2003-03-11 | GSE336 | GEO

The effects of SNAI2 overexpression on the transcriptional program in HaCaT cells

Project description:Investigation of the transcriptional profile of SNAI2-overexpressing HaCaT cells.

2021-09-01 | GSE166199 | GEO

RARa restrains CD8 T cell effector program [CHIP-seq]

Project description:RARa restrains CD8 T cell effector program [CHIP-seq]

| PRJNA1072639 | ENA