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Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions

ABSTRACT: Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions

PROVIDER: PRJNA929495 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23272246_1.fastq.gz	Fastqsanger.gz
	SRR23272247_1.fastq.gz	Fastqsanger.gz
	SRR23272248_1.fastq.gz	Fastqsanger.gz
	SRR23272249_1.fastq.gz	Fastqsanger.gz
	SRR23272250_1.fastq.gz	Fastqsanger.gz

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Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions

Project description:Actinobacillus pleuropneumoniae is the cause of porcine pleuropneumonia, a severe respiratory tract infection that is responsible for major economic losses to the swine industry. Many host-adapted bacterial pathogens encode systems known as phasevarions (phase-variable regulons). Phasevarions result from variable expression of cytoplasmic DNA methyltransferases. Variable expression results in genome-wide methylation differences within a bacterial population, leading to altered expression of multiple genes via epigenetic mechanisms. Our examination of a diverse population of A. pleuropneumoniae strains determined that Type I and Type III DNA methyltransferases with the hallmarks of phase variation were present in this species. We demonstrate that phase variation is occurring in these methyltransferases, and show associations between particular Type III methyltransferase alleles and serovar. Using Pacific BioSciences Single-Molecule, Real-Time (SMRT) sequencing and Oxford Nanopore sequencing, we demonstrate the presence of the first ever characterised phase-variable, cytosine-specific Type III DNA methyltransferase. Phase variation of distinct Type III DNA methyltransferase in A. pleuropneumoniae results in the regulation of distinct phasevarions, and in multiple phenotypic differences relevant to pathobiology. Our characterisation of these newly described phasevarions in A. pleuropneumoniae will aid in the selection of stably expressed antigens, and direct and inform development of a rationally designed subunit vaccine against this major veterinary pathogen.

2023-02-02 | GSE224057 | GEO

SWATH-MS proteome of Actinobacillus pleuropneumoniae showing differential protein expression due to the ModP and ModQ phasevarions

Project description:Actinobacillus pleuropneumoniae is responsible for porcine pleuropneumoniae, a severe respiratory disease that causes major global financial losses to the swine industry every year. Phase variation is the random switching of gene expression, either ON-OFF switching of expression, or the expression of multiple allelic protein variants. A number of bacteria encode cytoplasmic DNA methyltransferases that are phase-variable. This variable methyltransferase expression leads to global gene expression differences mediated by epigenetic mechanisms. These systems are called phasevarions (phase-variable regulons). In all described cases, phasevarions control expression of current and putative vaccine candidates, and influence phenotypes relevant to pathobiology. We have discovered two new phase-variable Type III DNA methyltransferases in A. pleuropneumoniae, which we named modP and modQ. Sequence analysis of multiple strains showed modP was present as four allelic variants, but just a single allelic variant of modQ was present. Phase-variable expression of modP (modP1 or modP2) and modQ leads to differential protein expression. This SWATH-MS dataset demonstrates the resulting changes to protein expression as a result of the of ModP1, ModP2 and ModQ phasevarions. This dataset represents triplicate repeats from strains which express either ModP or ModQ (ON) and strains which do not (OFF).

2023-05-10 | PXD036273 | Pride

Actinobacillus pleuropneumoniae BHI vs Koromycin

Project description:Actinobacillus pleuropneumoniae is the etiologic agent of contagious pleuropneumonia, an economically important disease of commercially reared swine throughout the world. To cause this disease, A. pleuropneumoniae must rapidly overcome porcine pulmonary innate immune defenses. Effects of koromycin, an antimicrobial agent that acts as an noncompetitive inhibitor of the interaction of NQR with its quinone substrate, on the transcriptome of A. pleuropneumoniae was investigated.

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Transcriptome of 6h static biofilm Actinobacillus pleuropneumoniae

Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

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Transcriptome of 4h static biofilm Actinobacillus pleuropneumoniae

Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

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Project description:Actinobacillus pleuropneumoniae Assembly

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Genome-wide screening and characterization of Actinobacillus pleuropneumoniae and its ciprofloxacin-resistant mutants

Project description:Understanding constraints which shape antibiotic resistance is key for predicting and controlling drug resistance. Here, we performed high-throughput laboratory evolution of Actinobacillus pleuropneumoniae and its ciprofloxacin resistance-inducing derivatives.This study aims to explore the mechanism of acquired ciprofloxacin resistance in Actinobacillus pleuropneumoniae.

2021-10-06 | GSE181030 | GEO

Transcript profiling of Actinobacillus pleuropneumoniae in BALF

Project description:Actinobacillus pleuropneumoniae is the etiologic agent of contagious pleuropneumonia, an economically important disease of commercially reared swine throughout the world. To cause this disease, A. pleuropneumoniae must rapidly overcome porcine pulmonary innate immune defenses. Since bronchoalveolar fluid (BALF) contains many of the innate immune components found in the lung, we examined the gene expression of a virulent serovar 1 strain of A. pleuropneumoniae after exposure to concentrated BALF. This experiment was also carried out with a malT mutant of the same strain.

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