Ontology highlight
ABSTRACT:
PROVIDER: PRJNA930173 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR23313055_1.fastq.gz | Fastqsanger.gz | |||
| SRR23313055_2.fastq.gz | Fastqsanger.gz | |||
| SRR23313056_1.fastq.gz | Fastqsanger.gz | |||
| SRR23313056_2.fastq.gz | Fastqsanger.gz | |||
| SRR23313057_1.fastq.gz | Fastqsanger.gz |
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Project description:Verticillium dahliae is a soil-borne fungus with a broad host range, including the model plants Nicotiana benthamiana and Arabidopsis thaliana. The plant immunity can be activated by Vd-derived patterns while V. dahliae secreted effectors. We report here the RNAseq analyses of samples from N. benthamiana infected by V. dahliae strains 592 and 171 at different time points. The data showed dynamic expression patterns of genes not only from plant defense signaling but also plant developmental signaling.
2023-07-13 | GSE236729 | GEO
Project description:Verticillium dahliae is a soil-borne vascular pathogen that causes severe wilt symptoms in a wide range of plants. Co-culture of the fungus with Arabidopsis roots for 24 hours induces many changes in the gene expression profiles of both partners, even before defense-related phytohormone levels are induced in the plant. Both partners reprogram sugar and amino acid metabolism, activate genes for signal perception and transduction, and induce defense and stress responsive genes. Furthermore, analysis of Arabidopsis expression profiles suggests a redirection from growth to defense. The plant and fungal genes that rapidly respond to the presence of the partner might be crucial for early recognition steps and the future development of the interaction. Thus they are potential targets for the control of V. dahliae-induced wilt diseases.
2018-02-08 | GSE104590 | GEO
Project description:Verticillium dahliae is a soilborne fungus that causes wilt disease in plants. The microsclerotia of V. dahliae produce infectious hyphae that give rise to primary infections. In this study, RNA-seq libraries were prepared from microsclerotia (MS)-producing cultures of V. dahliae (ave = 52.23 million reads), and those not producing microsclerotia (NoMS, ave = 50.58 million reads) and analyzed for differential gene expression.
2013-09-06 | E-MTAB-1581 | biostudies-arrayexpress
Project description:To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in Arabidopsis, we generated small RNA sequences from adult Arabidopsis plants under control and under dought, salt, and cold stress treatments. Over 23 million reads were generated.
2012-03-12 | GSE26356 | GEO
Project description:The soilborne fungus, Verticillium dahliae, causes Verticillium wilt disease in plants. Verticillium wilt is difficult to control since V. dahliae is capable of persisting in the soil for 10 to15 years as melanized microsclerotia, rendering crop rotation strategies for disease control ineffective. Microsclerotia of V. dahliae overwinter and germinate to produce infectious hyphae that give rise to primary infections. Consequentially, microsclerotia formation, maintenance, and germination are critically important processes in the disease cycle of V. dahliae.
2013-09-06 | E-MTAB-1793 | biostudies-arrayexpress
Project description:Global gene expression was compared between Arabidopsis lines with altered expression of ANAC102 (over-expressed and knocked-out) and wild-type. ANAC102 is a putative NAC domain transcription factor. Gene expression was compared between an ANAC102 over-expressing line and parental ecotype C24 under ambient atmosphere to determine which genes ANAC102 is capable of regulating. Gene expression was also compared between three week old plants of an ANAC102 knock-out line and parental ecotype Col-0 under 0.1% Oxygen and ambient atmosphere conditions to determine which genes may require ANAC102 for appropriate expression under these conditions. Gene expression was also compared between imbibed seeds of an ANAC102 knock-out line and parental ecotype Col-0 following a 0.1% Oxygen treatment. Keywords: Genetic modification
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Project description:Verticillium dahliae Kleb., a soil-borne fungus that colonizes vascular tissues, induces wilting, chlorosis and early senescence in potato. Difference in senescence timing found in two diploid potato clones, 07506-01 and 12120-03, was studied and genetic variation in response to V. dahliae infection was identified as a causal factor. The clone, 07506-01, was infected with V. dahliae but did not develop symptoms, indicating tolerance to the pathogen. The other diploid clone, 12120-03 had low levels of pathogen with infection and moderate symptoms indicating partial resistance. 07506-01 was found to carry two susceptible alleles of the Ve2 gene and 12120-03 carried one Ve2 resistant and one susceptible allele. Infected leaves of the two clones were compared using gene expression profiling with the Potato Oligonucleotide Chip Initiative (POCI) microrarray. The results provide further evidence for differences in response of the two clones to infection with V. dahliae. Chlorophyll biosynthesis was higher in the tolerant 07506-01 compared to partially resistant 12120-03. On the other hand, expression of fungal defense genes, Ve resistance genes and defense phytohormone biosynthetic enzyme genes was decreased in 07506-01 compared to 12120-03 suggesting defense responses were suppressed in tolerance compared to resistance. Transcription factor gene expression differences pointed to the WRKY family as potential regulators of V. dahliae responses in potato. Two-color microarray comparison of two clones, three biological replicates of each clone, one dye swap technical replicate
2013-07-15 | E-GEOD-48881 | biostudies-arrayexpress
Project description:The V. dahliae could cause host obvious disease symptoms. Moreover, it was difficult to control, because of the rapidly genetic variation. Due to the rapidly genetic variation and complexity of the genetic background, the mechanism of pathogenicity differentiation of V. dahliae has still been unknown. To solve the problem, we isolated 24 strains of V. dahliae from a farmland of Shihezi city, Xinjiang province. Through ITS sequences and VCGs assay, the strains of SHZ-4, SHZ-5, and SHZ-9 had closer kinship. Moreover, the large difference was presented on the pathogenicity of SHZ-4, SHZ-5, and SHZ-9. Furthermore, a number of differentially expressed genes was identified through comparative transcriptome sequencing method and the genes were mainly centered on CWDEs and transport relative proteins. It was well understand that the CWDEs determined the source of nutrients of V. dahliae and the organic substance of host cell degraded by the CWDEs would induce the hyperosmosis of intercellular liquid. According to these, we proposed a hypothesis that the hyperosmosis induced the dehydration and death of host cell and contributed to absorb the nutrition for V. dahliae. The hyperosmosis promoting the nutrients scramble indicated that the V. dahliae could resist the hyperosmosis and the pathogenicity was positive associating with the tolerance. With regards to this, the sugar-induced-hyperosmotic resistance of the three V. dahliae isolates and cotton leaf tissue were analyzed through changing the sugar concentration. The result was entirely consistent with our hypothesis. It was firstly found that the pathogenicity of V. dahliae was relative to the resistance of sugar-induced-hyperosmosis, which could provide a new idea for cultivating a new varieties to resistant to the V. dahliae.
2019-10-25 | GSE131467 | GEO
Project description:Over-expression of bHLH122 in Arabidopsis
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