Brackish Caspian Sea Metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA931128 | ENA |
REPOSITORIES: ENA
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Project description:we used next-generation sequencing technology to characterise mRNA-seq of brackish water (BW, 10‰), fresh water (FW, 0‰), and sea water (SW, 25‰)-treated Anguilla marmorata's gill, kidney and intestine to elucidate the molecular mechanisms of salinity adaptation.
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Project description:Caspian Sea - Southern basin Raw sequence reads
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Project description:The Baltic Sea is one of the largest brackish water bodies in the world. Redoxclines that form between oxic and anoxic layers in the deepest sub-basins are a semi-permanent character of the pelagic Baltic Sea. The microbially mediated nitrogen removal processes in these redoxclines have been recognized as important ecosystem service that removes large proportion of the nitrogen load originating from the drainage basin. However, nitrification, which links mineralization of organic nitrogen and nitrogen removal processes, has remained poorly understood. To gain better understanding of the nitrogen cycling in the Baltic Sea, we analyzed the assemblage of ammonia oxidizing bacteria and archaea in the central Baltic Sea using functional gene microarrays and measured the biogeochemical properties along with potential nitrification rates. Overall, the ammonia oxidizer communities in the Baltic Sea redoxcline were very evenly distributed. However, the communities were clearly different between the eastern and western Gotland Basin and the correlations between different components of the ammonia oxidizer assemblages and environmental variables suggest ecological basis for the community composition. The more even community ammonia oxidizer composition in the eastern Gotland Basin may be related to the constantly oscillating redoxcline that does not allow domination of single archetype. The oscillating redoxcline also creates long depth range of optimal nitrification conditions. The rate measurements suggest that nitrification in the central Baltic Sea is able to produce all nitrate required by denitrification occurring below the nitrification zone.
2013-08-24 | GSE50164 | GEO
Project description:How species genetically adapt to new environments is a central question in evolutionary biology. Here whole-genome sequencing combined with functional analysis is used to dissect how Atlantic herring, a marine fish, has adapted to the brackish Baltic Sea. Genes involved in reproduction and early development emerge as primary targets of natural selection, with key changes in a sperm-specific anion channel (LRRC8C2), a zona pellucida protein (ZPBA1), a cluster of three genes for fish transglutaminase (FTG1-3), and a copy number expansion of a fish hatching enzyme gene (HE1C). The large diameter of LRRC8C2 homomers facilitates transport of ions and osmolytes, likely preventing swelling of sperm when spawning in low salinity. Altered ZPBA1 sequence together with modified FTG1-3 enzyme activity produces a harder egg envelope that prevents egg swelling in brackish waters, while the enhanced activity of the adapted HE1C enzyme enables larvae to digest this reinforced egg envelope during hatching. Baltic Sea herring populations reproducing in brackish water are fixed or nearly fixed for variant alleles at these four unlinked loci, each carrying multiple amino-acid substitutions compared to the alleles prevalent in the Atlantic Ocean populations. The alleles at three of these loci (ZPBA1, FTG1-3, and HE1C) have been introgressed from Pacific herring populations present in the Arctic Sea. These findings reveal concrete molecular mechanisms by which a marine species has adapted to a novel, low-salinity environment.
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2017-06-23 | GSE47400 | GEO
Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
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Project description:IL10-/-DC pulsed for 6h with 0, SEA, LPS, or co-pulsed with SEA/LPS together to compare changes in LPS-induced gene expression mediated by SEA (Schistosome soluble egg antigen) Keywords: other
2004-05-14 | GSE1382 | GEO