Project description:Epithelial-to-mesenchymal transition is a complex process that occurs in development and cancer metastasis. We show that EMT plasticity occurs in cancer cells when co-culture with fibroblasts. We used microarrays to detail the global program of gene expression in the H69 small-cell lung carcinoma line when in direct contact with CCD8 fibroblasts.
Project description:To examine the impact of TP53 knockout in cancer-associated fibroblasts on the progression of liver cancer, we conducted a study wherein cancer-associated fibroblasts were collected and subjected to TP53 knockout. Subsequently, the cell supernatants were harvested and analyzed for alterations in protein expression utilizing proteomic methodologies.