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Global gene expression of post-senescent telomerase negative ter1Δ strain of Ustilago maydis

ABSTRACT: Global gene expression of post-senescent telomerase negative ter1Δ strain of Ustilago maydis

PROVIDER: PRJNA935941 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23498211_1.fastq.gz	Fastqsanger.gz
	SRR23498211_2.fastq.gz	Fastqsanger.gz
	SRR23498212_1.fastq.gz	Fastqsanger.gz
	SRR23498212_2.fastq.gz	Fastqsanger.gz
	SRR23498213_1.fastq.gz	Fastqsanger.gz

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Global gene expression of post-senescent telomerase negative ter1Δ strain of Ustilago maydis

Project description:The dimorphic fungus Ustilago maydis transits from unicellular sporidia to multicellular mycelium and accomplish its life cycle in planta with teliospore formation. Mutations in either of telomerase core subunits, trt1 or ter1 cause deficiencies or inability to produce galls and teliospores when crossed to a wild-type strain. Here, the global transcriptome analysis of two ter1Δ survivor strains of U. maydis is reported. It revealed the deregulation of many telomerase-deleted response (TDR) genes previously reported in other telomerase-negative fungi, as the DDR and stress response genes, cell cycle, subtelomeric and some telomere proximal genes. Interestingly, other differentially expressed genes (DEGs) found in those ter1Δ survivors were pathogenic lifestyle factors, plant-pathogen crosstalk, iron uptake, meiosis, and melanin synthesis. The studied survivors were phenotypically comparable, yet they exhibited DEGs between them. Our global results suggest the teliospore formation in U. maydis controlled by key genes of pathogenic lifestyle and meiosis

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Telomerase RNA subunit of Ustilago maydis

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Project description:The RNA subunit of telomerase is an essential component whose primary sequence and length are poorly conserved among eukaryotic organisms. The phytopathogen Ustilago maydis is a dimorphic fungus of the Ustilaginaceae family. We analyzed several members of the Ustilaginaceae family to computationally identify the TElomere RNA (TER) gene ter114. To confirm the identity of the TER gene, we disrupted the gene and characterized telomerase-negative mutants. Similar to catalytic TERT mutants, ter114Δ mutants exhibit phenotypes of telomere shortening and low replicative potential. However, ter114Δ mutants had very prolonged replication times, and survivors arose more rapidly in ter114Δ (252 replication rounds) than in their counterpart trt1Δ mutant strains (300 replication rounds). ter114-disrupted mutants were unable to infect maize seedlings in heterozygous crosses and showed defects such as cell cycle arrest and segregation failure. We concluded that ter114, which encodes the TER subunit of the telomerase of U. maydis, could have distinct or more extensive functions than trt1. To further characterize the ter114 gene, we analyze the transcriptomes of both, mRNA nuclear transcripts and nuclear RNA ribo-depleted transcripts of the wild-type strain 521, from the reads of both nuclear fractions, we find four reconstructed transcripts of the ter114 locus, which constitute four isoforms containing the structural domains of TER. The results showed that the transcription start site for the four isoforms is located upstream the start codon of the uncharacterized gene UMAG_03168, while the differential expression analysis showed that the ter114-i4 isoform is up-regulated with respect to the polyadenylated fraction, suggesting that this transcript could correspond to the precursor of the mature form of TER.

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Project description:We analyzed the global expression patterns of telomerase-negative mutants from haploid cells of Ustilago maydis to identify the gene network required for cell survival in the absence of telomerase. Mutations in either of the telomerase core subunits (trt1 and ter1) of the dimorphic fungus U. maydis cause deficiencies in teliospore formation. We report the global transcriptome analysis of two ter1Δ survivor strains of U. maydis, revealing the deregulation of telomerase-deleted responses (TDR) genes, such as DNA-damage response, stress response, cell cycle, subtelomeric, and proximal telomere genes. Other differentially expressed genes (DEGs) found in the ter1Δ survivor strains were related to pathogenic lifestyle factors, plant-pathogen crosstalk, iron uptake, meiosis, and melanin synthesis. The two ter1Δ survivors were phenotypically comparable, yet DEGs were identified when comparing these strains. Our findings suggest that teliospore formation in U. maydis is controlled by key pathogenic lifestyle and meiosis genes.

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