Project description:Abstract: As part of the zebrafish genome annotation project the 3 prime ends of genes were pulled down on polyT beads and sequenced on the Illumina Genome Analyzer to identify alternative 3 prime ends in a range of tissues and developmental stages. <br> Study description: Total RNA from a range of developmental stages and adult tissues were chemically fragmented, pulled down on polyT magnetic beads and double stranded cDNA was synthesized. The cDNA was BpmI digested to release from the beads and to leave a 6 T base tail. Illumina sequencing libraries were made followed by 76 base paired-end sequencing.
Project description:gnp07_regeneome_cuc2 - cuc2 - CUC2 is expressed in meristem. It permits to create organs boundaries. It is also expressed in leave margins. Is there a mecanism meristem like in leave margins? - To compare wt and cuc2 leaf margins. And compare teeth and hollow inside the arabidopsis leaf margin.