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ABSTRACT: Manure bacteria

PROVIDER: PRJNA939438 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23654646_1.fastq.gz	Fastqsanger.gz
	SRR23654646_2.fastq.gz	Fastqsanger.gz
	SRR23654647_1.fastq.gz	Fastqsanger.gz
	SRR23654647_2.fastq.gz	Fastqsanger.gz
	SRR23654648_1.fastq.gz	Fastqsanger.gz

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Steroids originating from bacterial bile acid degradation affect Caenorhabditis elegans and indicate potential risks for the fauna of manured soils

Project description:Bile acids are steroid compounds from the digestive tracts of vertebrates that enter agricultural environments in unusual high amounts with manure. Bacteria degrading bile acids can readily be isolated from soils and waters including agricultural areas. Under laboratory conditions, these bacteria transiently release steroid compounds as degradation intermediates into the environment. These compounds include androstadienediones (ADDs), which are C19-steroids with potential hormonal effects. Experiments with Caenorhabditis elegans showed that ADDs derived from bacterial bile acid degradation had effects on its tactile response, reproduction rate, and developmental speed. Additional experiments with a deletion mutant as well as transcriptomic analyses revealed that these effects might be conveyed by the putative testosterone receptor NHR-69. Soil microcosms showed that the natural microflora of agricultural soil is readily induced for bile acid degradation accompanied by the transient release of steroid intermediates. Establishment of a model system with a Pseudomonas strain and C. elegans in sand microcosms indicated transient release of ADDs during the course of bile acid degradation and negative effects on the reproduction rate of the nematode. This proof-of-principle study points at bacterial degradation of manure-derived bile acids as a potential and so-far overlooked risk for invertebrates in agricultural soils.

2019-02-08 | GSE126214 | GEO

Project description:Calf manure bacteria transplantation metagenome

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Isolation of Antibiotic-Resistant Bacteria from the Manure of Free-Range Chickens (Gallus domesticus): Implications for Zoonotic Transmission

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Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm

Project description:Meta-proteomics analysis approach in the application of biogas production from anaerobic digestion has many advantages that has not been fully uncovered yet. This study aims to investigate biogas production from a stable 2-stage chicken manure fermentation system in chemical and biological perspective. The diversity and functional protein changes from the 1st stage to 2nd stage is a good indication to expose the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explain the causation of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, the hydrogenotrophic methanogenic pathway is adopted as indicative of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomic analysis, this study could provide both species names of microorganism and enzymes to directly point the generation pathway of methane and carbon dioxide in investigating biogas production of chicken manure.

2021-02-08 | PXD022498 | Pride

Transcriptome analysis of potato tubers: Effects of different agricultural practices

Project description:The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments. Keywords: experiment with factorial design factorial design; 2 potato cultivars (Sante, Lady Balfour); 2 fertilizers (dairy manure compost, chicken manure pellets); 3 plant protection treatments (copper oxychloride, comcat, water), 3 biological replicates, 48 samples

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Project description:Soil and manure-related bacteria genome sequencing and assembly

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Protein expression profile in composting habitat

Project description:Metaproteomics analysis of the evolution of protein expression profile during cattle manure composting

2025-01-07 | PXD059527 |

Project description:anaerobic co-degestion of straw and cow manure, bacteria sequence

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Project description:Prairie strip removal of swine manure associated bacteria from runoff

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