Project description:To evaluate the role of PHF2 in skeletal muscle, we generated skeletal muscle conditional Phf2 KO mice and analyzed the transcriptome by CUT&RUN-sequencing.
Project description:To evaluate the role of PHF2 in skeletal muscle, we generated skeletal muscle conditional Phf2 KO mice and analyzed the transcriptome by CUT&RUN-sequencing.
Project description:To evaluate the role of PHF2 in skeletal muscle, we generated skeletal muscle conditional Phf2 KO mice and analyzed the transcriptome by RNA-sequencing.
Project description:To model and characterize the chromatin regulatory landscape in Neural stem cell before and after Phf2 knockout, we performed HiC to map large-scale 3D architectural rewiring in WT and Phf2-KO NSC.
Project description:The establishment of C2C12 cell line with knockout of TAOK1 expression using CRISPR/Cas9 system. C2C12 cells with TAOK1 knockout were designated as C2C12-TAOK1-KO, while those transfected with an empty vector served as the control group (C2C12-Mock), non-transfected cells served as the blank group (C2C12-Blank). The C2C12-Blank, C2C12-Mock and C2C12-TAOK1-KO cells (10000 cells/mL) were seeded into 100 mm cell culture dishes and induced to differentiation into myotubes. Then, cells were obtained by trypsin digestion and centrifugation. Samples from three independent experiments were collected.