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Proteomic analysis of TAOK1 knockout in C2C12 myotubes

ABSTRACT: The establishment of C2C12 cell line with knockout of TAOK1 expression using CRISPR/Cas9 system. C2C12 cells with TAOK1 knockout were designated as C2C12-TAOK1-KO, while those transfected with an empty vector served as the control group (C2C12-Mock), non-transfected cells served as the blank group (C2C12-Blank). The C2C12-Blank, C2C12-Mock and C2C12-TAOK1-KO cells (10000 cells/mL) were seeded into 100 mm cell culture dishes and induced to differentiation into myotubes. Then, cells were obtained by trypsin digestion and centrifugation. Samples from three independent experiments were collected.

ORGANISM(S): Mus Musculus

SUBMITTER: Xuan Liu

PROVIDER: PXD057089 | iProX | Wed Oct 23 00:00:00 BST 2024

REPOSITORIES: iProX

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