Project description:Microarray analysis of the gill tissues of WSSV infected shrimp (P. monodon) at different time intervals 6 hrs, 24 hrs, 48 hrs and moribund stage of post WSSV infection was carried out to identify differentially expressed genes in response to WSSV infection. The shrimps in WSSV challenege experiment were challenged through intra muscular route with known concentration of virus. The important immune genes identified would be further characterized by sequence analysis and gene expression profile would be validated by real time PCR
Project description:To evaluate the differential potential affected by SMARCE1 -MD/MD(R42A) , we performed RNA-sequencing (RNA-seq) of Smarce1-MD and control Smarce1-MD (R42A) embrynoic body.
Project description:Microarray analysis of the gill tissues of WSSV infected shrimp (P. monodon) at different time intervals 6 hrs, 24 hrs, 48 hrs and moribund stage of post WSSV infection was carried out to identify differentially expressed genes in response to WSSV infection. The shrimps in WSSV challenege experiment were challenged through intra muscular route with known concentration of virus. The important immune genes identified would be further characterized by sequence analysis and gene expression profile would be validated by real time PCR One-color experiment,Organism: Penaeus monodon, Custom Penaeus monodon (Black Tiger Shrimp) 8x60k designed by Genotypic Technology Private Limited (AMADID: 041733), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:The phenomenon of trained immunity, which facilitates vaccine development for disease control, has been identified in shrimp; however, the mechanism remains elusive. In the present study, we found that histone H3K27 acetylation (H3K27ac) mediated by the lysine acetyltransferase KAT8 plays an important role in preventing white spot syndrome virus (WSSV) infection in the shrimp Marsupenaeus japonicus. We then successfully established a model of trained immunity via the use of UV-inactivated WSSV to explore the underlying mechanism(s) in shrimp. In UV-WSSV-trained shrimp, the glycolysis and tricarboxylic acid (TCA) cycle metabolic pathways were enhanced and acetyl-CoA concentrations were increased. As the acetyl group donor, acetyl-CoA promotes KAT8 activity to increase H3K27 acetylation. H3K27ac is deposited at the promoter region of the transcription factor Dorsal to facilitate its expression and then Dorsal promotes the expression of an interferon-like cytokine, Vago5, and antimicrobial peptides that act against WSSV infection. H3K27ac is also deposited at the promoter region of hexokinase 2 and isocitrate dehydrogenase, which positively regulates glycolysis and the TCA cycle in a feedforward manner. Our results reveal a novel mechanism of trained immunity induced by UV-WSSV in shrimp and provide a theoretical basis for the development of antiviral vaccines for disease control in shrimp aquaculture.