Project description:Total RNA was purified from keratinocytes isolated from FFPE arsenic-induced skin lesion samples collected from individuals exposed to high concentrations of arsenic exceeding 50 ppb in drinking water in Murshidibad district of West Bengal, India.
Project description:Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent public health threat, according to the CDC. This pathogen has few treatment options and causes severe nosocomial infections with >50% fatality rate. Although previous studies have examined the proteome of CRAb, there have been no focused analyses of dynamic changes to β-lactamase expression that may occur due to drug exposure. Here, we present our initial proteomic study of variation in β-lactamase expression that occurs in CRAb with different β-lactam antibiotics. Briefly, drug resistance to Ab (ATCC 19606) was induced by the administration of various classes of β-lactam antibiotics, and the cell-free supernatant was isolated, concentrated, separated by SDS-PAGE, digested with trypsin, and identified by label-free LC-MS-based quantitative proteomics. Thirteen proteins were identified and evaluated using a 1789 sequence database of Ab β-lactamases from UniProt, the majority of which were Class C β-lactamases (≥80%). Importantly, different antibiotics, even those of the same class (e.g. penicillin and amoxicillin), induced non-equivalent responses comprising various isoforms of Class C and D serine-β-lactamases, resulting in unique resistomes. These results open the door to a new approach of analyzing and studying the problem of multi-drug resistance in bacteria that rely strongly on β-lactamase expression.
Project description:Dissemination of various NDM-type metallo-beta-lactamases producing multidrug-resistant Escherichia coli clinical isolates in Nepal
| PRJDB5173 | ENA
Project description:Antibiotic resistant & beta-lactamases producing Gram-negative bacterial isolate from wastewater of Himachal Pradesh, India
| PRJNA1421069 | ENA
Project description:Multidrug resistant & beta-lactamases producing Gram-negative bacteria isolated from wastewater of Himachal Pradesh, India
| PRJNA1421061 | ENA
Project description:Bacterial metataxonomy of agricultural soil sample of North Bengal, West Bengal, India
| PRJNA733696 | ENA
Project description:Bacterial diversity of agricultural soil sample of North Bengal, West Bengal, India