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Gene expression profiling of NUP98-HOXA9 expressing mouse embryonic stem cells

ABSTRACT: Gene expression profiling of NUP98-HOXA9 expressing mouse embryonic stem cells

PROVIDER: PRJNA944118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23828940.fastq.gz	Fastqsanger.gz
	SRR23828941.fastq.gz	Fastqsanger.gz
	SRR23828942.fastq.gz	Fastqsanger.gz
	SRR23828943.fastq.gz	Fastqsanger.gz

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Gene expression profiling of NUP98-HOXA9 expressing mouse embryonic stem cells

Project description:To investigate the effect of NUP98-HOXA9 on global gene expression, we established FLAG-NUP98-HOXA9 expressing mouse embryonic stem cell lines.

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Expression data from murine hematopoietic cells expressing BCR-ABL alone, NUP98-HOXA9 alone, BCR-ABL and NUP98-HOXA9, or null for both oncogenes

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Project description:Rearrangements involving the NUP98 gene resulting in fusions to several partner genes occur in acute myeloid leukemia and myelodysplastic syndromes. This study demonstrates that the second FG repeat domain of the NUP98 moiety of the NUP98-HOXA9 fusion protein is important for its cell immortalization and leukemogenesis activities. We demonstrate that NUP98-HOXA9 interacts with MLL via this FG repeat domain and that, in the absence of MLL, NUP98-HOXA9-induced cell immortalization and leukemogenesis are severely inhibited. Molecular analyses indicate that MLL is important for the recruitment of NUP98-HOXA9 to the HOXA locus and for NUP98-HOXA9-induced HOXA gene expression. Our data indicate that MLL is crucial for NUP98-HOXA9 leukemia initiation.

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