Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A temporal extracellular transcriptome atlas of human preimplantation development

ABSTRACT: A temporal extracellular transcriptome atlas of human preimplantation development

PROVIDER: PRJNA945056 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A temporal extracellular transcriptome atlas of human preimplantation development

Project description:We develop a non-invasive method for comprehensive characterization of the extracellular RNAs (exRNA) in a single droplet of spent media that was used to culture human in vitro fertilization (IVF) embryos. We generate and provide as a resource the temporal extracellular transcriptome atlas of human preimplantation development (TETA). TETA consists of 245 exRNA sequencing datasets, corresponding to 245 collections of spent media at five developmental stages.

2023-11-14 | GSE227442 | GEO

An atlas of small non-coding RNAs in Human Preimplantation Development

Project description:An atlas of small non-coding RNAs in Human Preimplantation Development

| PRJNA1050210 | ENA

Single-cell identification of L1HS-Ta retrotransposons in preimplantation embryos by ATLAS-seq

Project description:We used ATLAS-seq to comprehensively map the genomic location of LINE-1 elements belonging to the youngest and potentially polymorphic subfamily (L1HS-Ta). This was performed in single-cells of 2 preimplantation embryos (E3 and E6) as well as from the remaining inner cell mass (denoted T). In brief, single cells were isolated from the inner cell mass of preimplantation embryos by laser drilling and micromanipulation. Whole-genome Multiple Displacement Amplification was performed on each isolated single cells, as well as on the remaining cells of the inner cell mass as a population (samples labelled 'T'). Then we applied ATLAS-seq to map L1HS-Ta retrotransposons. This approach relies on the random mechanical fragmentation of the genomic DNA to ensure high-coverage, ligation of adapter sequences, suppression PCR-amplification of L1HS-Ta element junctions, and Ion Torrent sequencing using single-end 400 bp read chemistry. A notable aspect of ATLAS-seq is that we can obtain both L1 downstream and upstream junctions (3'- and 5'-ATLAS-seq libraries, respectively), for full-length L1 elements.

2023-09-30 | E-MTAB-8616 | biostudies-arrayexpress

An atlas of small non-coding RNAs in Human Preimplantation Development [Small-Seq]

Project description:An atlas of small non-coding RNAs in Human Preimplantation Development [Small-Seq]

| PRJNA1050220 | ENA

An atlas of small non-coding RNAs in Human Preimplantation Development [RNA-seq]

Project description:An atlas of small non-coding RNAs in Human Preimplantation Development [RNA-seq]

| PRJNA1049378 | ENA

Transcriptome of mouse preimplantation development

Project description:Transcriptome of mouse preimplantation development

| PRJNA291206 | ENA

Comparative proteomic landscapes provide insights into mammalian preimplantation development and failure

Project description:Understanding mammalian preimplantation development, particularly in humans, at the proteomic level remains limited. Here, we applied our comprehensive solution of ultrasensitive proteomic technology to measure the proteomic profiles of oocytes and early embryos and identified nearly 8,000 proteins in humans and over 6,300 proteins in mice. We observed distinct proteomic dynamics before and around zygotic genome activation (ZGA) between the two species. Integrative analysis with translatomic data revealed extensive divergence between translation activation and protein accumulation. Multi-omic analysis indicated that ZGA transcripts often contribute to protein accumulation in blastocysts. Using mouse embryos, we identified several transcriptional regulators critical for early development, thereby linking ZGA to the first lineage specification. Furthermore, single-embryo proteomics of poor-quality embryos from over 100 patient couples provided insights into preimplantation development failure. Our study may contribute to reshaping the framework of mammalian preimplantation development and opening avenues for addressing human infertility.

2025-02-05 | PXD054371 | Pride

An atlas of small non-coding RNAs in Human Preimplantation Development (Mouse embryo RNA-seq)

Project description:An atlas of small non-coding RNAs in Human Preimplantation Development (Mouse embryo RNA-seq)

| PRJNA1138744 | ENA

Comparative proteomic landscapes provide insights into mammalian preimplantation development

Project description:Proteins play a crucial role in the central dogma as the end product of gene expression. However, a deep proteomic landscape is still the missing puzzle piece of the comprehensive understanding of mammalian preimplantation development. Here, using our recently developed Comprehensive Solution of Ultrasensitive Proteome Technology (CS-UPT), we measured the proteomic profiles of oocytes and early embryos for ~5000 proteins in humans and over 6200 proteins in mice. Comparative analysis identified divergent proteomic dynamics before and around zygotic genome activation (ZGA) between the two species. Integrative analysis with transcriptome and translatome data revealed extensive and stage-specific protein turnover events. Remarkably, our comprehensive multi-omics analysis indicates that ZGA transcripts significantly contribute to protein accumulation at the blastocyst stage. Additionally, we experimentally confirmed several pf these key regulators are essential for early mouse development. Our data provide a rich resource for mammalian preimplantation development and laid a solid foundation for future investigations.

2025-11-27 | PXD043363 | Pride

Transcription profiling of preimplantation mouse embryo development

Project description:Preimplantation mouse embryo development study on Affymetrix MOE430A. Zeng et al (2004) Dev Biol 272:483-496.

2008-06-11 | E-GEOD-1744 | biostudies-arrayexpress