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The phage-encoded protein PIT2 impacts bacterial quorum sensing in Pseudomonas aeruginosa by direct interaction with LasR

ABSTRACT: The phage-encoded protein PIT2 impacts bacterial quorum sensing in Pseudomonas aeruginosa by direct interaction with LasR

PROVIDER: PRJNA946327 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23908633_1.fastq.gz	Fastqsanger.gz
	SRR23908633_2.fastq.gz	Fastqsanger.gz
	SRR23908634_1.fastq.gz	Fastqsanger.gz
	SRR23908634_2.fastq.gz	Fastqsanger.gz
	SRR23908635_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

The phage-encoded protein PIT2 impacts bacterial quorum sensing in Pseudomonas aeruginosa by direct interaction with LasR

Project description:Quorum sensing (QS) is the cell density-dependent virulence factor regulator in Pseudomonas aeruginosa. Here, we elucidate PIT2, a phage-encoded inhibitor of the QS regulator LasR, derived from the lytic Pseudomonas phage LMA2. PIT2 inhibits the effectors PrpL and LasA of the type 2 secretion system of P. aeruginosa and attenuates bacterial virulence towards HeLa cells and in Galleria mellonella. Using RNAseq-based differential gene expression analysis, the effect of PIT2 on the LasR regulatory network was revealed. Moreover, the specific interaction between LasR and PIT2 was determined. These data expand our knowledge on phage-encoded modulators of the bacterial metabolism, as this examples an anti-virulence protein derived from a lytic phage. From an applied perspective, this phage protein reveals and exploits an interesting anti-virulence target in P. aeruginosa. As such, it lays the foundation for a new phage-inspired anti-virulence strategy to combat multidrug resistant pathogens and opens the door for SynBio applications.

2023-09-27 | GSE227677 | GEO

Transcription profiling of wild type, lasR rhIR and rpoS Pseudomonas aeruginosa mutants treated with acyl-HSL to investigage quorum sensing during the logarithmic phase of growth

Project description:Effect of acyl-HSL signal or ectopic lasR, rhlR, or rpoS expression on the advancement of quorum sensing gene expression during the logarithmic phase of growth

2007-08-10 | E-MEXP-1183 | biostudies-arrayexpress

Expression profile for PA14 lasR rhlR double mutant with H2O2 stress

Project description:Quorum sensing controls the expression of multiple virulence factors. PA14 genes lasR and rhlR are necessary for quorum sensing via homoserine lactones. A PA14 lasR rhlR deficient mutant exhibits a reduced oxidative stress response. Here we conducted a microarray to determine oxidative stress response gene regulation mediated by the homoserine lactone quorum sensing circuits. A PA14 lasR rhlR deficient mutant was compared to the wild-type with and without H2O2 stress.

2015-01-09 | E-GEOD-54245 | biostudies-arrayexpress

Expression profile for PA14 lasR rhlR double mutant with H2O2 stress

2015-01-09 | GSE54245 | GEO

A Distinct QscR Regulon in the Pseudomonas aeruginosa Quorum Sensing Circuit

2006-01-18 | GSE4026 | GEO

Peptide-based quorum sensing systems in Paenibacillus polymyxa

Project description:Paenibacillus polymyxa is an agriculturally important plant growth promoting rhizobacterium (PGPR). Many Paenibacillus species are known to be engaged in complex bacteria-bacteria and bacteria-host interactions, which in other bacteria were shown to necessitate quorum sensing communication, but to date no quorum sensing systems have been described in Paenibacillus. Here we show that the type strain P. polymyxa ATCC 842 encodes at least 16 peptide-based communication systems. Each of these systems comprises a pro-peptide that is secreted to the growth medium and further processed to generate a mature short peptide. Each peptide has a cognate intracellular receptor of the RRNPP family, and we show that external addition of P. polymyxa communication peptides to the medium leads to reprogramming of the transcriptional response. We found that these quorum sensing systems are conserved across hundreds of species belonging to the Paenibacillaceae family, with some species encoding more than 25 different peptide-receptor pairs, representing a record number of quorum sensing systems encoded in a single genome.

2020-08-05 | PXD015319 | Pride

Saccharomyces cerevisiae - Quorum Sensing

Project description:Comparison of 3 yeast strains grown under nitrogen starvation, nitrogen rich conditions, with and without quorum sensing molecule 2-phenylethanol

2025-07-08 | PXD061716 | JPOST Repository

Pseudomonas aeruginosa lasR mutants in young cystic fibrosis patients

Project description:Pseudomonas aeruginosa undergoes genetic change during chronic infection of the airways of cystic fibrosis (CF) patients. One common change is mutation of lasR. LasR is a transcriptional regulator that responds to one of the quorum sensing signals in P. aeruginosa, and regulates acute virulence factor expression as well as central metabolic functions. P. aeruginosa mutants in which lasR was inactivated emerged in the airways of CF patients early during chronic infection, and during growth in the laboratory on Luria-Bertani agar. Both environments are rich in amino acids. Inactivation of lasR in these isolates conferred a growth advantage with amino acids, a phenotype that could account for selection of lasR mutants both in vivo and in vitro. P. aeruginosa lasR mutants were identified by their distinctive colony morphology, including autolysis that correlated with an imbalance in 4-hydroxy-2-alkylquinolines (HAQs), and an iridescent metallic sheen likely caused by the accumulation of one such HAQ. The alterations in transcriptional profile due to inactivation of lasR were conserved in isolates from multiple young CF patients. P. aeruginosa lasR mutations may represent surrogate markers to delineate stages in the natural history of CF airway disease, each with different prognostic and therapeutic implications, analogous to the markers used to direct cancer treatment. Similar to cancer cell mutations that promote unrestricted growth, lasR mutations may promote unrestricted growth of P. aeruginosa in the CF airway by enabling more efficient utilization of available amino acids. Analyse the effects of mutation of the lasR gene in Pseudomonas aeruginosa isolates from cystic fibrosis patients by comparing the transcriptional profile of an isolate from a young patient with that of an isogenic engineered lasR mutant.

2010-06-30 | E-GEOD-5560 | biostudies-arrayexpress

Transcription profiling of Pseudomonas aeruginosa Quorum Sensing Circuit

Project description:To better understand the role of QscR in P. aeruginosa gene regulation and to better understand the relationship between QscR, LasR and RhlR control of gene expression we used transcription profiling to identify a QscR-dependent regulon. Our analysis revealed that QscR activates some genes and represses others. Some of the repressed genes are not regulated by the LasR-I or RhlR-I systems while others are. The LasI-generated 3-oxododecanoyl-homoserine lactone serves as a signal molecule for QscR. Thus QscR appears to be an integral component of the P. aeruginosa quorum sensing circuitry. QscR uses the LasI-generated acyl-homoserine lactone signal and controls a specific regulon that overlaps with the already overlapping LasR and RhlR-dependent regulons. Experiment Overall Design: First comparisaon Experiment Overall Design: We first compared transcriptomes of the qscR mutant P. aeruginosa PAO-R3 and the parental strain PAO1 at several points during growth (OD600nm 0.5, 0.8, 1.4, 2.0 and 3.5). To identify those genes with expression significantly different between PAO1 and PAO-R3 at different culture densities we used CYBER-T. The Bayesian prior estimate was 10 and the sliding window size was 101. The p-value threshold was 0.001, the posterior probability of differential expression >0.95, and fold change was >2.5. Experiment Overall Design: Second comparison Experiment Overall Design: We selected genes that were at least 3-fold differentially expressed in the strain containing the L-arabinose promoter-driven qscR allele vs the strain containing L-arabinose promoter-driven qscR-Ddbd allele, and were also at least 3-fold differentially expressed in the parent strain PAO1 as compared to the strain containing the qscR null mutation. RNA were extracted from cultures at OD 0.5, 0.8, 1.4, 2.0 and 3.5

2008-06-12 | E-GEOD-4026 | biostudies-arrayexpress

Pseudomonas aeruginosa lasR QS network promotes phage infection by regulating the biosynthesis of LPS and type IV pili by regulating the biosynthesis of LPS and type IV pili

Project description:Purpose: The purpose of this study was to investigate the effect of quorum sensing on phage infection. Methods: We constructed the lasR gene knockout strain of Pseudomonas aeruginosa PAO1 and performed transcriptome sequencing.

2024-04-08 | GSE246747 | GEO

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