Bos taurus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA94687 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The liver of dairy cows naturally displays a series of metabolic adaptation during the periparturient period in response to the increasing nutrient requirement of lactation. The hepatic adaptation is partly regulated by insulin resistance and it is affected by the prepartal energy intake level of cows. We aimed to investigate the metabolic changes in the liver of dairy cows during the periparturient at gene expression level and to study the effect of prepartal energy level on the metabolic adaptation at gene expression level.B13:N13
2017-10-05 | GSE97437 | GEO
Project description:Hepatic molecular adaptations underlying periparturient metabolic diseases such as ketosis in dairy cows are largely unknown. We used a simple model for induction of ketosis to examine liver gene expression profiles using a microarray consisting of 13,257 annotated cattle oligonucleotides. At 4 days post-partum, 7 cows classified as healthy after a physical examination were fed at 50% of intake at day 4 from day 5 to signs of ketosis or until 14 days post-partum. Another group of 7 healthy cows served as controls. Liver was biopsied at 9-14 (ketosis) or 14 days post-partum (controls). More than 9,000 sequences represented on the microarray were expressed in liver. Keywords: disease state analysis
2007-10-09 | GSE4304 | GEO
Project description:Protein profile analysis of the muscle tissue of ketosis dairy cows. Analyzing the changing trends of key proteins in the muscle tissue of ketosis dairy cows after the onset of ketosis.
| PXD066580 |
Project description:The objective of this experiment was to determine transcriptional differences in the visceral (VAT) and subcutaneous adipose tissue (SAT) of dairy cows with or without subclinical ketosis.
2025-07-09 | GSE245350 | GEO
Project description:The aim of this study was to determine the effects of linseed dietary supplementation on gene expression in the mammary gland of grazing dairy cows. Milk composition and gene expression in the mammary gland tissue were evaluated in dairy cows supplemented with linseed. The linseed supplementation improves the health and nutrition quality aspects of dairy milk, but also affects the gene networks expression signature associated with cellular growth and proliferation, cell-death, signalling, nutrient metabolism, and immune response, and in turn, the mammary gland integrity and health.
2012-11-18 | GSE24200 | GEO
Project description:Mammary transcriptome analysis of lactating dairy cows
| PRJNA106193 | ENA
Project description:Prepartum body condition score and plane of nutrition affect the hepatic transcriptome during the transition period in grazing dairy cows
| PRJNA323499 | ENA
Project description:Liver plays a profound role in the acute phase response (APR) observed in the early phase of acute bovine mastitis caused by Escherichia coli (E. coli). To gain an insight into the genes and pathways involved in hepatic APR of dairy cows we performed a global gene expression analysis of liver tissue sampled at different time points before and after intra-mammary (IM) exposure to E. coli lipopolysaccharide (LPS) treatment. Experiment Overall Design: Eight healthy, high yielding Holstein-Friesian dairy cows in their first lactation (9 to 12 weeks after calving) were chosen for this study. At time 0 the right front quarter was infused with 200 μg E. coli LPS dissolved in 10 ml 0.9% NaCl solution, the left front quarter serving as control was infused with 10 ml 0.9% NaCl solution. Liver biopsies were taken at â22, 3, 6, 9, 12 and 48 hours relative to LPS infusion in 4 cows, and also at â22, 9 and 48 hours in the remaining 4 cows. RNA from liver biopsies was isolated and biotin labeled cRNA was loaded onto the Affymetric GeneChip Bovine Genome Array. A control study using cows infused with 0.9% NaCl showed that there was no effect of taking the biopsy, neither in the clinical measurement nor in the expression of a selected subset of genes. Therefore, only samples taken from the LPS treated cows were measured for the gene expression using microarrays.
2008-11-13 | E-GEOD-10695 | biostudies-arrayexpress
Project description:Excessive body fat mobilization during the transition period overwhelms hepatic fatty acid (FA) oxidative capacity, leading to increased ketone body production and ketosis in dairy cows. Skeletal muscle (SM) has been shown to uptake and oxidize FA via mitochondrial β-oxidation and may play a crucial role in counteracting the hepatic FA overload. However, the role of SM in the pathogenesis of ketosis remains unclear. The objective of this study was to investigate transcriptomic alterations in the SM of postpartum dairy cows diagnosed with ketosis and identify genes and pathways potentially involved in the pathogenesis of the disease. Multiparous Holstein cows within 7 days in milk (DIM) were classified based on their blood β-hydroxybutyrate (BHB) concentrations as ketosis (BHB ≥ 1.3 mmol/L and reduced rumination; n = 15) and controls (BHB ≤ 1.1 mmol/L with no clinical signs; n = 15). On the day of enrollment, a biopsy from the external oblique muscle was collected and later processed for RNA sequencing. Differential expression analysis identified 3,543 differentially expressed genes (DEG), with 1,722 upregulated and 1,821 downregulated in the ketosis group relative to controls. Transcriptomic signatures of SM during ketosis revealed the upregulation of genes involved in protein synthesis, folding, and degradation, alongside downregulation of genes involved in mitochondrial energy production, extracellular matrix synthesis and remodeling, and muscle structure and contraction. In addition, our findings suggest impaired uptake and utilization of ketone bodies by SM in ketotic cows, as evidenced by the reduced expression of several genes involved in ketone catabolism. Although these findings suggest the involvement of SM in ketosis, further studies are warranted to determine whether these alterations contribute to the development and progression of ketosis or arise as a consequence of the disease. Keywords: ketosis, ketone bodies, RNA-Seq, skeletal muscle, muscle metabolism
2026-08-21 | GSE344020 | GEO