Project description:BALF and plasma proteomic profiling from the Clinical Response in Pneumonia Therapy (SCRIPT) study which aims to improve treatment strategies for patients with severe pneumonia. The samples were obtained during the early stages of the COVID-19 pandemic (June 15th, 2018 - JulClinical Response in Pneumonia Therapy (SCRIPT) study which aims to improve treatment strategies for patients with severe pneumonia. The samples were obtained during the early stages of the COVID-19 pandemic (June 15th, 2018 - July 6th, 2020) and included patients facing respiratory failure requiring mechanical ventilation in the intensive care unit (ICU).
Project description:Description:
Mass spec raw data in association with Sudhakar JN, et al in Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas, Nat Comms (accepted 2020 July).
Project description:Paper: Transcriptional Profiling of Plasmodium falciparum Parasites from Patients with Severe Malaria Identifies Distinct Low vs. High Parasitemic Clusters Milner et al Plos One July 18, 2012
Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing
Project description:Twelve inshore and six offshore colonies were reciprocally transplanted during 1 year (July 2017- July 2018) at Florida Keys (location). After this period samples were collected from the field and brought to the Experimental Reef Laboratory facilities (RSMAS, Miami) to be acclimated to 30C during 7 days in six aquaria. Three aquaria were keep under initial conditions for the duration of the experiment (30C) and three aquaria had the temperature increased everyday during 7 days to a final temperature of 32C. A total of 56 samples were collected for RNAseq after 6 days of the temperature treatment and stored at -80C.
Project description:<p>Gestational diabetes mellitus (GDM) is a common complication of pregnancy, particularly affecting multiparous women with a history of GDM, who face a recurrence risk approaching 50%. This study investigates the association between gut microbiota modifications and metabolic profiles from early to mid-pregnancy and the recurrence of GDM in a cohort of 70 multiparous women with prior GDM. Conducted as a prospective cohort study at Beijing Obstetrics and Gynecology Hospital from July 2018 to December 2020, participants underwent assessments including oral glucose tolerance tests (OGTT) and collection of fecal and serum samples. Microbial DNA was extracted, with the V3-V4 region of the 16S rRNA gene sequenced, while serum metabolites were analyzed through non-targeted metabolomics using mass spectrometry. Results indicated significant alterations in gut microbiota composition, with specific genera associated with increased GDM risk. Notably, the study identified distinct metabolic patterns, revealing downregulation of several key metabolites in recurrent GDM cases compared to controls. Pathway enrichment analysis highlighted dysregulation in metabolic functions pertinent to serotonergic synapses, arachidonic acid metabolism, unsaturated fatty acid biosynthesis, and caffeine metabolism. Overall, this research underscores the critical role of gut microbiota and metabolic changes in understanding GDM recurrence, providing insights that may inform targeted preventive strategies.</p>