Project description:Temperate perennial plants have to adapt their growth in order to survive over winter conditions and protect their tissues by entering into dormancy. The dormancy phenomenon is a complex process regulated by many factors, externals (photoperiod and temperature) or internals through the transport towards the buds. In order to better understand the molecular mechanisms involved in the regulation of dormancy, we conducted a transcriptomic analysis of flower buds from the late flowering sweet cherry cultivar 'Regina' in controlled conditions with a cold deprivation treatment under short days (day length of 8h) and in field conditions from July 2018 to June 2019 and repeat the cold deprivation treatment but in long days (day length of 16h) from July 2020 to June 2021. Moreover, to complement this experiment in order to explore the response to temperature in this opposite way, we performed a cold exposure from July 2023 and March 2024 in long days (day length of 16h).
Project description:BALF and plasma proteomic profiling from the Clinical Response in Pneumonia Therapy (SCRIPT) study which aims to improve treatment strategies for patients with severe pneumonia. The samples were obtained during the early stages of the COVID-19 pandemic (June 15th, 2018 - JulClinical Response in Pneumonia Therapy (SCRIPT) study which aims to improve treatment strategies for patients with severe pneumonia. The samples were obtained during the early stages of the COVID-19 pandemic (June 15th, 2018 - July 6th, 2020) and included patients facing respiratory failure requiring mechanical ventilation in the intensive care unit (ICU).
Project description:Description:
Mass spec raw data in association with Sudhakar JN, et al in Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas, Nat Comms (accepted 2020 July).
2020-07-27 | MSV000085831 | MassIVE
Project description:Oakley Mountain soil sample in July of 2018
Project description:Changes in sensory gene expression may underly seasonal changes in behavior. To accompany other research on seasonal changes in coloration and behavior in the seasonally plastic common buckeye butterfly Junonia coenia, we examined how gene expression in eyes differs between wild butterflies caught in summer and fall. We collected animals in 2019, 2020, and 2021 in the central United States. The samples consisted of three males and three females from each season: Summer (June/July) and Fall (late September/October) that had wing colors appropriate for previously described seasonal morphs (i.e. light in summer and dark in fall). We dissected eye tissue from butterfly heads and purified RNA prior to sending samples to Novogene for cDNA Library prep and RNA-Sequencing. We used DESeq2 for subsequent analysis. We found gene expression correlated to sex, season, and interactions between sex and season. Eye tissues from dark fall animals and light summer animals exhibit different patterns of expression in many genes including visual system development genes, pigmentation genes, and clock genes. This change in gene expression accompanies changes in basking behavior and wing color and indicates that J. coenia undergoes changes to its visual system depending on the seasonal environment.