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Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [miRNA-seq]

ABSTRACT: Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [miRNA-seq]

PROVIDER: PRJNA967678 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24445817.fastq.gz	Fastqsanger.gz
	SRR24445818.fastq.gz	Fastqsanger.gz
	SRR24445819.fastq.gz	Fastqsanger.gz
	SRR24445820.fastq.gz	Fastqsanger.gz
	SRR24445821.fastq.gz	Fastqsanger.gz

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Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [RNA-seq]

Project description:Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [RNA-seq]

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Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [RNA-seq]

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Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [miRNA-seq]

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Transcription profiling of lung and bronchial lymph node from mice infected with Respiratory Syncytial Virus (RSV)

Project description:Transcriptional responses in lungs of mice infected with Respiratory Syncytial Virus (RSV) were compared to a control and mock infections

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Respiratory syncytial virus (RSV) enhances translation of virus-resembling AU-rich host transcripts

Project description:Viral infection often triggers eukaryotic initiator factor 2α (eIF2α) phosphorylation, leading to global 5’-cap-dependent translation inhibition. RSV encodes messenger RNAs (mRNAs) mimicking 5’-cap structures of host mRNAs and thus inhibition of cap-dependent translation initiation would likely also reduce viral translation. We confirmed that RSV limits widespread translation initiation inhibition and unexpectedly found that the fraction of ribosomes within polysomes increases during infection, indicating higher ribosome loading on mRNAs during infection. We found that AU-rich host transcripts that are less efficiently translated under normal conditions become more efficient at recruiting ribosomes, similar to RSV transcripts. Viral transcripts are transcribed in cytoplasmic inclusion bodies, where the viral AU-rich binding protein M2-1 has been shown to bind viral transcripts and shuttle them into the cytoplasm. We further demonstrated that M2-1 is found on polysomes, and that M2-1 might deliver host AU-rich transcripts for translation.

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