Project description:Chinese mitten crab (Eriocheir sinensis) is one of the most popular bred crustaceans in China. E. sinensis stressed by high temperature and resulted in disease and large mortality in summer. Litter is known about the mechanism of temperature tolerant E. sinensis. In this study, transcriptome and proteome for high temperature tolerant (HTT) and high temperature sensitive (HTS) female E. sinensis were identified
Project description:The accessory gland (AG), an important component of the male reproductive system, enhances the fertility of spermatozoa during male reproduction. Some AG proteins are known to bind to the spermatozoa membrane and affect its function and properties. In this study, we describe, for the first time, a comprehensive catalog of proteins of the AG secreted fluid during the mature phase of the Chinese mitten crab (Eriocheir sinensis). AG secreted proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS).
Project description:Reference genome was assembed for mixture sample of Eriocheir sinensis eyestalk under low tempeture (7°C,12°C,17°C) ,normal temperature (22°C) and high temperature (27°C, 32°C) conditions.The reference genome was used for the further transcritome analysis of genes expression profiels in eystalk of Eriocheir sinensis under different tempetures and provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results.
