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The Gene Profile of Senescent Corneal Cells

ABSTRACT: The Gene Profile of Senescent Corneal Cells

PROVIDER: PRJNA972477 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24554740_1.fastq.gz	Fastqsanger.gz
	SRR24554740_2.fastq.gz	Fastqsanger.gz
	SRR24554741_1.fastq.gz	Fastqsanger.gz
	SRR24554741_2.fastq.gz	Fastqsanger.gz
	SRR24554742_1.fastq.gz	Fastqsanger.gz

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The Gene Profile of Senescent Corneal Cells

Project description:To investigate the gene profiles of senescent corneal cells by X-ray exposure.

2023-10-25 | GSE232508 | GEO

The Gene Profile of Senescent Conjunctival Cells

Project description:The Gene Profile of Senescent Conjunctival Cells

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Gene expression profile of senescent and non-senescent cells from regenerating blastemas

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Gene expression profile of the SV40-immortalized human corneal epithelial cells

2010-06-24 | GSE22539 | GEO

Gene expression profile of the SV40-immortalized human corneal epithelial cells

Project description:Microarray was used to study global gene expression of a cell culture model based on SV40-immortalized human corneal epithelial (iHCE) cells. The gene expression profile of the cell line was compared to the normal human corneal epithelium. Affymetrix HG-U133A GeneChipsÂ® were used for microarray experiments and results were validated by performing RT-qPCR for selected genes. iHCE was found to over- and under-express 22 % and 14 % of the annotated genes, respectively. The results of this study suggest that differences between iHCE cells and normal corneal epithelium are substantial and therefore the use of these cells in corneal research should be considered with caution. SV40-immortalized human corneal epithelial (iHCE) cells were cultured on collagen coated permeable supports for one week using standard culture conditions. Subsequently the apical medium was removed and culturing was continued at the air-liquid interface which allows cells to stratify. Trans-epithelial electrical resistance was followed and total RNA was extracted from cultures with resistance > 300 Î© x cm2. RNA was amplified, labeled with biotin, fragmented and hybridized to the HG-U133A GeneChipsÂ® (Affymetrix) according to the protocol of the manufacturer. Three separate experiments with samples from three independent culture batches (iHCE1, iHCE2, iHCE3) were performed. The Global gene expression profile of iHCE was compared to previously published human corneal epithelial tissue (GSE5543) data.

2010-07-29 | E-GEOD-22539 | biostudies-arrayexpress

Senescent Characteristics of Human Corneal Endothelial Cells upon Ultraviolet-A Exposure

Project description:Purpose: The objective of this study was to investigate the senescent phenotypes of human corneal endothelial cells (hCEnCs) upon treatment with ultraviolet (UV)-A. Methods: We assessed cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation and expression of senescence markers (p16 and p21) in hCEnCs exposed to UV-A radiation, and senescent hCEnCs induced by ionizing radiation (IR) were used as positive controls. We performed RNA sequencing and proteomics analysis to compare gene and protein expression profiles between UV-A- and IR-induced senescent hCEnCs, and we also compared the results to non-senescent hCEnCs. Results: Cells exposed to 5 J/cm2 of UV-A or to IR exhibited typical senescent phenotypes, including enlargement, increased SA-β-gal activity, decreased cell proliferation and elevated expression of p16 and p21. RNA-Seq analysis revealed that 83.9% of the genes significantly upregulated and 82.6% of the genes significantly downregulated in UV-A-induced senescent hCEnCs overlapped with the genes regulated in IR-induced senescent hCEnCs. Proteomics also revealed that 93.8% of the proteins significantly upregulated in UV-A-induced senescent hCEnCs overlapped with those induced by IR. In proteomics analyses, senescent hCEnCs induced by UV-A exhibited elevated expression levels of several factors part of the senescence-associated secretory phenotype. Conclusion: In this study, where senescence was induced by UV-A, a more physiological stress for hCEnCs compared to IR, we determined that UV-A modulated the expression of many genes and proteins typically altered upon IR treatment, a more conventional method of senescence induction, even though UV-A also modulated specific pathways unrelated to IR.

2024-05-17 | GSE250224 | GEO

Senescent Characteristics of Human Corneal Endothelial Cells upon Ultraviolet-A Exposure

Project description:Senescent Characteristics of Human Corneal Endothelial Cells upon Ultraviolet-A Exposure

| PRJNA1052700 | ENA

Gene expression of senescent and non-senescent AL1 cells

Project description:Gene expression of senescent and non-senescent AL1 cells

| PRJNA849785 | ENA

The Gene Profile of Senescent Conjunctival Cells

Project description:To investigate the gene profiles of senescent conjunctival cells by X-ray exposure.

2023-10-25 | GSE237091 | GEO

Expression data from human corneal endothelial cells

Project description:Human corneal endothelial cells (HCEC) form a monolayer by adhering tightly through their intercellular adhesion molecules. Located at the posterior corneal surface, they maintain corneal translucency by dehydrating the corneal stroma, mainly through the Na+- and K+-dependent ATPase (Na+/K+-ATPase). Because HCEC proliferative activity is low in vivo,we tried to activate proliferation of HCEC by inhibiting cyclin-dependent kinase inhibitors.We have here demonstrated microarray data of transduced human corneal endothelial cell lines. Affymetrix human U133 plus 2.0 array was used to transcriptionally profile to compare cultured human corneal endothelial cells and transduced human corneal endothelial cells.

2011-10-29 | E-GEOD-30781 | biostudies-arrayexpress

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