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Scalable production of human cortical organoids using a biocompatible polymer

ABSTRACT: Scalable production of human cortical organoids using a biocompatible polymer

PROVIDER: PRJNA972757 | ENA |

REPOSITORIES: ENA

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Scalable production of human cortical organoids using a biocompatible polymer

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Human cortical organoids. Chronic treatment with GSK3 inhibitor CHIR99021. Bulk RNAseq.

Project description:Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.).Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 18, day 50, day 100).

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