Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNAseq gene expression and VDJ-TCR analysis of PBMCs from patients with tattoo uveitis and sarcoidosis

ABSTRACT: scRNAseq gene expression and VDJ-TCR analysis of PBMCs from patients with tattoo uveitis and sarcoidosis

PROVIDER: PRJNA973988 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24641256.fastq.gz	Fastqsanger.gz
	SRR24641257.fastq.gz	Fastqsanger.gz
	SRR24641258.fastq.gz	Fastqsanger.gz
	SRR24641259.fastq.gz	Fastqsanger.gz
	SRR24641260.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

scRNAseq gene expression and VDJ-TCR analysis of PBMCs from patients with tattoo uveitis and sarcoidosis

Project description:To elucidate the complexity of immune responses in the formation of granuloma, we performed the single-cell RNA-sequencing analysis. PBMCs derived from tattoo uveitis patients in both active and inactive stages and sarcoidosis patients were collected to characterize their similarities and difference. PBMCs from age-matched healthy donors were considered as controls.

2025-02-20 | GSE232802 | GEO

scRNAseq of aqueous immune cells in human uveitis

Project description:scRNAseq of aqueous immune cells in human uveitis

| PRJNA953326 | ENA

Transcriptome analysis of peripheral blood mononuclear cells in pulmonary sarcoidosis

Project description:Purpose: This study aimed to explore the pathobiological markers of sarcoidosis in PBMCs by comparing the transcriptional signature of PBMCs from patients with pulmonary sarcoidosis and those of healthy controls by RNA sequencing. Methods:PBMC samples were collected from subjects with pulmonary sarcoidosis with no steroid/immunosuppressant drugs (n = 8) and healthy controls (n = 11) from August 2020 to April 2021, and RNA sequencing was performed with the PBMC samples. Results: Variety of DEGs were determined between groups leading to the enrichment analysis. Conclusions: The present study demonstrated that bulk gene expression patterns in PBMCs were different between patients with pulmonary sarcoidosis and healthy controls. The changes in the gene expression pattern of PBMCs could reflect the existence of sarcoidosis lesions and influence granuloma formation in sarcoidosis.

2021-12-31 | GSE192829 | GEO

scRNAseq of aqueous immune cells in human uveitis

Project description:We profiled using single cell RNA sequencing the immune cells of the aqueous humor from patients with uveitis.

2023-12-14 | GSE229166 | GEO

Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis

Project description:Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins.

2017-01-31 | PXD005150 | Pride

scRNAseq of PBMCs from idiopathic multicentric Castleman Disease (iMCD) flare and remission

Project description:We performed scRNAseq of PBMCs from three idiopathic multicentric Castleman Disease (iMCD) patients with paired flare and remission samples

2020-05-20 | GSE140881 | GEO

Microarray study using whole blood and purified leucocyte populations from healthy controls and patients with tuberculosis or sarcoidosis with tuberculosis and sarcoidosis

Project description:Whole blood and purified leucocyte populations were compared to determine which cell types the whole blood interferon-inducible signature, found in both tuberculosis and sarcoidosis, was present in. This study agreed with previous publication that the neutrophil was the dominant cell in tuberculosis and also showed it was the dominant cell type in sarcoidosis. There were 5 patients in each group of healthy controls, tuberculosis and sarcoidosis. The cell types were whole blood, PBMCs, monocytes (CD14), T cells (CD8 and CD4) and neutrophils (CD15).

2013-10-31 | E-GEOD-42832 | biostudies-arrayexpress

Peripheral blood gene expression as a novel genomic biomarker in complicated sarcoidosis

Project description:Sarcoidosis, a systemic granulomatous syndrome invariably affecting the lung, typically spontaneously remits but in ~20% of cases progresses with severe lung dysfunction or cardiac and neurologic involvement (complicated sarcoidosis). Unfortunately, current biomarkers fail to distinguish patients with remitting (uncomplicated) sarcoidosis from other fibrotic lung disorders, and fail to identify individuals at risk for complicated sarcoidosis. We utilized genome-wide peripheral blood gene expression analysis to identify a gene signature distinguishing sarcoidosis from healthy controls, which also served as a molecular signature for complicated sarcoidosis. Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 29 African descent American (AA) and 10 European descent American (EA) patients with sarcoidosis were included in the overall sarcoidosis cohort with 18 AA and 4 EA patients diagnosed with complicated sarcoidosis.

2012-06-30 | E-GEOD-37912 | biostudies-arrayexpress

scRNAseq on sorted T-cells and CITE-seq on PBMCs

Project description:scRNAseq on sorted T-cells and CITE-seq on PBMCs

| PRJNA770555 | ENA

scRNAseq of the TCRs and BCRs of aqueous and blood immune cells in human uveitis

Project description:We profiled using single cell RNA sequencing the immune cells of the aqueous humor from patients with uveitis as well as their peripheral blood. Then processed their BCR repertoire and TCR repertoire

2025-01-02 | GSE274471 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data