Project description:Lower selection intensity resulted in obvious genetically and phenotypically divergences in China indigenous breeds. Nanyang black pig, a China indigenous breed, was famous for its high lipid deposition and high genetic divergence, which made it an ideal model investigating mechanism of lipid position traits in pig. Here, transcriptome and TMT-based proteome analyses were carried out in longissimus dorsi (LD) tissue of high genetic variation individual Nanyang black pigs. After phenotyping in a big population with multi-production traits indexes, six Nanyang black pigs were selected and divided into relatively high and low lipid deposition groups. Combining analyses of transcriptomic and proteomic data identified 15 candidate genes determining lipid deposition genetic divergence in Nanyang black pig. Among them, FASN, CAT, and SLC25A20 were main causal candidate genes. The other genes could be divided as lipid deposition related gene (BDH2, FASN, CAT, DHCR24, ACACA, GK, SQLE, ACSL4, SCD), PPARA-centered fat metabolism regulatory factors (PPARA, UCP3), transcription or translation regulators (SLC25A20, PDK4, CEBPA), and integrin, structural proteins, signal transduction-related genes (EGFR). The multi-omics data set provided a valuable resource for analyses on lipid deposition-traits in pig, especially in Nanyang black pig.
Project description:To evaluate the roles of miRNA in porcine liver, dynamic profiles of microRNAome were investigated in swine breeds with different traits of commercial interest, we sampled liver tissues from a Chinese well-known elite native breed of Enshi black pig, a Large White pig, and a Chinese wild boar living within the same environment at the same day-old(90d).
Project description:To explore the methylome differences of swine breeds with different traits of commercial interest, we sampled liver tissues from a Chinese well-known elite native breed of Enshi black pig, a Large White pig, and a Chinese wild boar living within the same environment at the same daya-old (90d).
Project description:To evaluate the roles of gene regulation in porcine liver, dynamic profiles of transcriptome were investigated in swine breeds with different traits of commercial interest, we sampled liver tissues from a Chinese well-known elite native breed of Enshi black pig, a Large White pig, and a Chinese wild boar living within the same environment at the same day-old(90d).
Project description:<p>Neonatal mortality is mostly due to a reduced piglet maturity. An essential question for adaptation to extra-uterine environment lies in the optimization of the crosstalk between maternal and fetal genomes for a distribution of resources that allows fetuses to express healthier phenotypes at birth. The CO-LOcATION project proposes an integrative biology approach combining transcriptomics, metabolomics, lipidomics and maturity phenotypes to answer this crucial question for both research and pig production sustainability. The project will use samples from the former ANR PORCINET project, which produced pure genotypes and reciprocal crossbreed genotypes with contrasted robustness at birth. The complexity of the maturity phenotypes will be addressed by studying the trade-off between growth and survival with a focus on endometrial-placental interactions using whole-genome approaches.</p>