Project description:To investigate matrix gla protein (Mgp) function in angiogenesis and adipogenesis in brown adipose tissue (BAT) of C57BL/6 mice. we dissected 5 pairs of BAT from 4 weeks male Mgp wild-type (WT) and knock out (KO) mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of 5 pairs of WT and KO mice.
Project description:The integrity of the vascular wall is crucial for maintaining the normal function of blood vessels. Matrix Gla protein (MGP), originally identified as an inhibitor of bone morphogenetic proteins (BMPs) and vascular calcification, is now recognized as a key vascular morphogen that guides cell differentiation across various tissues. In this study, we employed an MGP Pro64Gly knock-in mouse model alongside single-cell RNA sequencing to explore the role of MGP in mediating vascular cell differentiation. The Pro64Gly mutation specifically impairs MGP's ability to bind to and inhibit BMPs.
Project description:Gene expression profile from brown adipose tissues of Prdm16 knockout and wile type mice. Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development.
Project description:One-pot enrichment and label-free quantification of protein acetylation and protein succinylation in mouse brown adipose tissue (BAT) in response to cold-acclimation and/or BAT-specific Sirt5 KO.
Project description:Gene expression profile from brown adipose tissues of Prdm16 knockout and wile type mice. Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. Brown adipose tissues were collected from Prdm16 knockout and wiletype mice with 4 biological replicates per condition. Experiment was done in two separate batch for 6-week-old and 11-month-old. Extracted RNA was hybridized to Agilent two-color arrays.
Project description:This project includes proteomic data of brown adipose tissue from mice modeled with Cuprizone 300mg/Kg/Day for one month versus wild-type mice. CB represents the brown adipose tissue from wild type mice. MB represents the brown adipose tissue from mice treated with cuprizone.
Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Keywords: tissue comparison from mice