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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia

ABSTRACT: Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia

PROVIDER: PRJNA978030 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24781000.fastq.gz	Fastqsanger.gz
	SRR24781001.fastq.gz	Fastqsanger.gz
	SRR24781002.fastq.gz	Fastqsanger.gz
	SRR24781003.fastq.gz	Fastqsanger.gz
	SRR24781004.fastq.gz	Fastqsanger.gz

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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia [spatial transcriptomics]

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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia [snRNA-Seq]

Project description:We performed spatial and single-cell transcriptomics on male mouse brains to map out the molecular and cellular processes governing the response to ischemic stroke during the first week after the injury. Focusing on glial cells, we documented their activation, the formation of the glial scar, and significant changes in cell–cell communication patterns. Interestingly, we identified various activated populations of oligodendrocytes that, while similar in their anti-inflammatory properties, differ in their immunogenic and metabolic characteristics. This challenges the long-held belief that oligodendrocytes play a passive role in neuropathologies. Altogether, we present a major contribution to the current understanding of the role of glial cells in stroke pathobiology, comprehensively mapping their acute response and role in the formation of the glial scar.

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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia.

Project description:The role of nonneuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling of the male mouse brain during the first week of injury. Cortical gene expression was severely disrupted, defined by inflammation and cell death in the lesion core, and glial scar formation orchestrated by multiple cell types on the periphery. The glial scar was identified as a zone with intense cell-cell communication, with prominent ApoE-Trem2 signaling pathway modulating microglial activation. For each of the three major glial populations, an inflammatory-responsive state, resembling the reactive states observed in neurodegenerative contexts, was observed. The recovered spectrum of ischemia-induced oligodendrocyte states supports the emerging hypothesis that oligodendrocytes actively respond to and modulate the neuroinflammatory stimulus. The findings are further supported by analysis of other spatial transcriptomic datasets from different mouse models of ischemic brain injury. Collectively, we present a landmark transcriptomic dataset accompanied by interactive visualization that provides a comprehensive view of spatiotemporal organization of processes in the postischemic mouse brain.

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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia

Project description:This SuperSeries is composed of the SubSeries listed below.

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