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Sciatic nerve crush regulation of RNA alternative poly-adenylation [DRGinjury_2019-QuantSeq_Rev_24h]

ABSTRACT: Sciatic nerve crush regulation of RNA alternative poly-adenylation [DRGinjury_2019-QuantSeq_Rev_24h]

PROVIDER: PRJNA978052 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24781176.fastq.gz	Fastqsanger.gz
	SRR24781177.fastq.gz	Fastqsanger.gz
	SRR24781178.fastq.gz	Fastqsanger.gz
	SRR24781179.fastq.gz	Fastqsanger.gz
	SRR24781180.fastq.gz	Fastqsanger.gz

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Sciatic nerve crush regulation of RNA alternative poly-adenylation

Project description:Poly-adenylation regulates post-transcriptional processing and localization of mRNA. To study the effect of nerve injury on poly-adenylation across the transcriptome, 3'-end RNAseq was carried on DRG neurons following sciatic nerve injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

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Sciatic nerve crush regulation of RNA alternative poly-adenylation [DRGinjury_2019-QuantSeq_Rev_24h]

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Project description:Sciatic nerve crush regulation of RNA alternative poly-adenylation

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Project description:To profile downstream gene-expression changes regulated by DLK, we profiled motoneurons in the lumbar spinal cord using RiboTag, following sciatic nerve crush in both control and Dlk conditional knockout (Dlk ΔMN) mice. We found that DLK regulates the expression of many secreted proteins, which have the potential to influence the behavior of other cells, including the immune system.

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