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Ngn family transcription factors reprogram mouse embryonic fibroblasts into neuron-like cells

ABSTRACT: Ngn family transcription factors reprogram mouse embryonic fibroblasts into neuron-like cells

PROVIDER: PRJNA982132 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24887276_1.fastq.gz	Fastqsanger.gz
	SRR24887276_2.fastq.gz	Fastqsanger.gz
	SRR24887277_1.fastq.gz	Fastqsanger.gz
	SRR24887277_2.fastq.gz	Fastqsanger.gz
	SRR24887278_1.fastq.gz	Fastqsanger.gz

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Ngn family transcription factors reprogram mouse embryonic fibroblasts into neuron-like cells

Project description:Ngn family transcription factors (TFs) are important neurogenic regulators during development. The aim of this study is to examine the ability of two Ngn family members, Ngn1 and Ngn3, to induce neuron fates from mouse embryonic fibroblasts (MEFs) through direct cell fate reprogramming.

2024-12-01 | GSE234613 | GEO

Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210

Project description:Neural stem cells (NSCs) are valuable for both basic research and clinical application. We previously reported a chemical cocktail that could reprogram somatic cells into neural progenitor cells. However, the process of chemical induction is complex and the underlying mechanism remains largely elusive. Here, we identified a new culture condition that greatly promotes the efficiency of NSC generation directly from mouse fibroblasts based on our reported chemical cocktail. Transcriptome and epigenome analyses during the reprogramming process demonstrated that growth factors including IL6, FGF5 and LIF were dynamically activated and contributed to the chemical cocktail induced cell fate changes. Interestingly, fibroblast-to-NSC conversion requires the synergistic action of these growth factors. Moreover, the reprogramming capacity of both chemical cocktail and growth factors require nucleoporin Nup210 to activate endogenous neural transcription factors SoxB1 family to initiate NSC fate. Our study not only provides a novel protocol to generate NSC directly from mouse fibroblasts, but also reveals an important role of growth factors in chemical-induced cellular reprogramming.

2019-12-31 | GSE108300 | GEO

Reprogram-seq

Project description:Reprogram-seq

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Transcription factors drive Tet2-mediated enhancer demethylation to reprogram cell fate

Project description:Transcription factors drive Tet2-mediated enhancer demethylation to reprogram cell fate

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Global gene expression in adult cardiac fibroblasts transduced with cardiac transcription factor

2012-04-06 | GSE37057 | GEO

Global gene expression in adult cardiac fibroblasts transduced with cardiac transcription factor

Project description:Four transcription factors, GATA4, Hand2, MEF2C, Tbx5 (GHMT) activated cardiac gene expression in cardiac fibroblasts, suggesting that these factors are able to reprogram fibroblasts toward a cardaic cell fate. Total RNA isolated from adult cardiac fibroblasts transduced with empty retroviral vector or GHMT-retroviruses for 2, and 4 weeks.

2012-04-05 | E-GEOD-37057 | biostudies-arrayexpress

Reprogramming fibroblasts into bi-potential hepatic stem cells by defined factors

Project description:Recent studies have demonstrated direct reprogramming of fibroblasts into a range of somatic cell types, but to date stem/progenitor cells have only been reprogrammed for the blood and neuronal lineages. We previously reported generation of induced hepatocyte-like (iHep) cells by transduction of Gata4, Hnf1α, and Foxa3 in p19 Arf null mouse embryonic fibroblasts (MEFs). Here, we show that Hnf1β and Foxa3, liver organogenesis transcription factors, are sufficient to reprogram MEFs into induced hepatic stem cells (iHepSCs). iHepSCs can be stably expanded in vitro and possess the potential of bi-directional differentiation into both hepatocytic and cholangiocytic lineages. In the injured liver of fumarylacetoacetate hydrolase (Fah)-deficient mice, repopulating iHepSCs become hepatocyte-like cells. They also engraft as cholangiocytes into bile ducts of mice with DDC-induced bile ductular injury. Lineage-conversion into bi-potential expandable iHepSCs provides a strategy to enable efficient derivation of both hepatocytes and cholangiocytes for use in disease modeling and tissue engineering. iHepSCs were converted form fibroblasts by transduction of Hnf1β and Foxa3. iHepSCs were induced to differentiate into hepatocyte-like cells and cholangiocytes in vitro. Totally, 9 samples including four clones of iHepSCS, one clone of LEPCs, two samples of MEFs and two samples of iHepSCs-derived cholangocytes were analyzed.

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c-Myc alone is enough to reprogram fibroblasts into functional macrophages [RNA-seq]

Project description:c-Myc alone is enough to reprogram fibroblasts into functional macrophages [RNA-seq]

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c-Myc alone is enough to reprogram fibroblasts into functional macrophages [scRNA-seq]

Project description:c-Myc alone is enough to reprogram fibroblasts into functional macrophages [scRNA-seq]

| PRJNA1150967 | ENA

Direct reprogramming of fibroblasts into antigen-presenting dendritic cells

Project description:Ectopic expression of transcription factors has been used to reprogram differentiated somatic cells toward pluripotency or to directly reprogram them to other somatic cell lineages. This concept has been explored in the context of regenerative medicine. Here, we set out to generate dendritic cells (DCs) capable of presenting antigens from mouse and human fibroblasts. By screening combinations of 18 transcription factors that are expressed in DCs, we have identified PU.1, IRF8, and BATF3 transcription factors as being sufficient to reprogram both mouse and human fibroblasts to induced DCs (iDCs). iDCs acquire a conventional DC type 1–like transcriptional program, with features of interferon-induced maturation. iDCs secrete inflammatory cytokines and have the ability to engulf, process, and present antigens to T cells. Furthermore, we demonstrate that murine iDCs generated here were able to cross-present antigens to CD8+ T cells. Our reprogramming system should facilitate better understanding of DC specification programs and serve as a platform for the development of patient-specific DCs for immunotherapy.

2018-12-07 | GSE103618 | GEO

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