Project description:RNA modifications are an important component of epigenetic regulation and play essential roles in embryonic development. Among these modifications, N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic messenger RNA, regulates diverse physiological and pathological processes. Increasing evidence suggests that dysregulated m6A methylation contributes to congenital malformations and reproductive system disorders. In this study, methylated RNA immunoprecipitation sequencing (MeRIP-seq) was performed on penile tissues collected from normal rats and a rat model of hypospadias to characterize the transcriptome-wide m6A methylation landscape associated with hypospadias. These data provide a resource for investigating the potential role of m6A RNA methylation in the molecular mechanisms underlying hypospadias.
Project description:Genome wide DNA methylation profiling of primary penile squamous cell carcinoma samples with lymphnode metastasis. The Illumina Infinium 450 K Human Methylation was used to obtain DNA methylation profiles in surgical frozen samples. Among the 25 samples, fifteen was infected with HPV, and the others were HPV negative.
Project description:Genome-wide DNA methylation profiles were determined on a set of fresh 44 bladder cancer tissues using normal blood as control. DNA amplicons were prepared using Differential Methylation Hybridization (DMH) method, subsequently hybridized on to the Agilent Human CpG island Microarray. The goal was to unravel the DNA methylation patterns in different subgropus of bladder cancer along with finding markers for progresssion and early diagnosis.
Project description:Genome wide DNA methylation profiling of tumor and surrounding healthy colonic mucosa from patients with colorectal carcinoma. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across 27,578 CpG loci covering 14,475 genes.