Project description:Homogenous staining regions (hsr) are cytogenetic representations of a gene amplification. They are found exclusively in tumour cells. We found a hsr in the human embryonic stem cell (hESC) line H14, this is the first report of a hsr in hESCs. FISH and CGH studies showed that the hsr was derived from chromosome 17p11.2. The gene expression analysis studies were performed to identify the genes upregulated due to the hsr by comparison the the hsr-contianing H14 cells with a karyotypically normal parent H14 cell line. Keywords: karyotypically abnormal hESC vs karyotypically normal hESC.
Project description:Homogenous staining regions (hsr) are cytogenetic representations of a gene amplification. They are found exclusively in tumour cells. We found a hsr in the human embryonic stem cell (hESC) line H14, this is the first report of a hsr in hESCs. FISH and CGH studies showed that the hsr was derived from chromosome 17p11.2. The gene expression analysis studies were performed to identify the genes upregulated due to the hsr by comparison the the hsr-contianing H14 cells with a karyotypically normal parent H14 cell line. Experiment Overall Design: H14 HSR positive embryonic stem cells and H14 HSR negative cells were hybridised to Affymetrix U133 Plus 2.0 expression arrays. Replicates were also run using H14 positive and H14 negative cells from different passages.
Project description:Transcription profiling by array to investigate doxorubicin-induced toxicity in human cardiomyocytes derived from embryonic stem cell line SA121
Project description:Transccription profiling by array of embryonic stem cell line 2TS22C and its derivative trophoblast stem cell line 22CEROH2-TSC1 with or without tetracycline-induced conditional knockout of Sox2
Project description:Transcription profiling of mouse embryonic stem cell line CGR8 during differentiation to mesenchymal stem cells and then adipocyte precursors
Project description:Expression data from undifferentiated human embryonic stem cells (hESC) and Day 3.5 mesodermal progenitor (CD326neg CD56+) population
