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Short-term depletion of nicotinic acetylcholine receptor subunits in Drosophila enterocytes

ABSTRACT: Short-term depletion of nicotinic acetylcholine receptor subunits in Drosophila enterocytes

PROVIDER: PRJNA988543 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25054715.fastq.gz	Fastqsanger.gz
	SRR25054716.fastq.gz	Fastqsanger.gz
	SRR25054717.fastq.gz	Fastqsanger.gz
	SRR25054718.fastq.gz	Fastqsanger.gz
	SRR25054719.fastq.gz	Fastqsanger.gz

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Short-term depletion of nicotinic acetylcholine receptor subunits in Drosophila enterocytes

Project description:Bulk RNAseq from whole Drosophila melanogaster guts expressing either mCherry-RNAi (control) or RNAi against nicotinic acetylcholine receptor subunits β1 and β3 (nAchRβ1 and nAchRβ3) under control of a enterocyte-specific driver to assess their role in barrier function.

2023-08-23 | GSE236069 | GEO

Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins

Project description:Description Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that present a target for insecticides. However, a better understanding of in vioreceptor subunit composition is needed for effective design of insecticides. Alpha-neurotxins are known as peptide toxins capable to block nAChRs by binding to its target subunits. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten nAChR subunit genes. We studied interactions of nAChRs to a snake toxin by using styrene maleic acid lipid particles (SMALPs) extraction method. For the null alleles which are impaired in locomotor activities we examined the binding of nAChRs incorporated into SMALPs to alpha bungarotoxin by employing mass spectrometry. We show native ligand interactions to Dalpha5, Dalpha6 and Dalpha7 subunits and reveal glycosylation sites of Dalpha5 and Dalpha7 by a glycoproteomic approach. Finally, we report the localization of fluorescent tagged Dalpha6 across nervous system development

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whole brains from nicotinic acetylcholine receptor subunit mutant mice and littermate controls

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RNA sequencing data describing transcriptional changes in aorta of ApoE-/- mice after alpha 7 nicotinic acetylcholine receptor (α7nAChR) stimulation.

Project description:This manuscript is a companion paper to Ulleryd M.U. et al (1). Data shown here include RNA sequencing data from whole aorta of ApoE-/- mice treated with the alpha 7 nicotinic acetylcholine receptor (α7nAChR) agonist AZ6983 for 8 weeks using subcutaneously implanted osmotic minipumps. 1. Ulleryd MA, Mjornstedt F, Panagaki D, Yang LJ, Engevall K, Gutierrez S, et al. Stimulation of alpha 7 nicotinic acetylcholine receptor (α7nAChR) inhibits atherosclerosis via immunomodulatory effects on myeloid cells Re-submitted. 2019.

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α7 nicotinic acetylcholine receptor signaling modulates the inflammatory phenotype of fetal brain microglia: first evidence of interference by iron homeostasis.

Project description:We sequenced mRNA from 7 microglia extracted from sheep fetuses exposed to LPS, agonist and antagonist of α7 nicotinic acetylcholine receptor to determine signaling pathway through modeling of α7 during inflammation.

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Alpha7 Nicotinic Acetylcholine Receptor Mediates Chronic Nicotine Inhalation-Induced Cardiopulmonary Dysfunction

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Nicotinic Acetylcholine Receptors Regulate Growth Hormone in Pituitary Somatotrophs of Tigers

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α7 Nicotinic Acetylcholine Receptor Regulates Macrophage-Mediated Neuroinflammation in Dry Eye Disease

Project description:α7 Nicotinic Acetylcholine Receptor Regulates Macrophage-Mediated Neuroinflammation in Dry Eye Disease

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Short-term depletion of acetylcholine synthesis in Drosophila enteroendocrine cells

Project description:Bulk RNAseq from whole adult Drosophila melanogaster guts expressing either mCherry-RNAi (control) or RNAi against ChAT (Choline acteyltransferase) under control of a enteroendocrine-specific driver to assess the role of epithelial acetylcholine production in intestinal barrier function.

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