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Chromatin-enriched RNAs expression profiling by high-throughput sequencing of T47D-MTVL human breast cancer cells

ABSTRACT: Chromatin-enriched RNAs expression profiling by high-throughput sequencing of T47D-MTVL human breast cancer cells

PROVIDER: PRJNA992564 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25195850_1.fastq.gz	Fastqsanger.gz
	SRR25195850_2.fastq.gz	Fastqsanger.gz
	SRR25195851_1.fastq.gz	Fastqsanger.gz
	SRR25195851_2.fastq.gz	Fastqsanger.gz

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Chromatin-enriched RNAs expression profiling by high-throughput sequencing of T47D-MTVL human breast cancer cells

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Gene expression by high-throughput sequencing of T47D-MTVL human breast cancer cells upon H1.2 knock-down

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Gene expression profiling by high-throughput sequencing of T47D-MTVL human breast cancer cells upon H1X knock-down

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Gene expression of T47D-MTVL human breast cancer cells growing in normal conditions

Project description:Basal gene expression of T47D-MTVL human breast cancer cells growing in normal conditions Breast cancer cells T47D-MTVL cells were grown in rich media and total RNA was extracted to monitor basal gene expression

2014-02-04 | E-GEOD-49236 | biostudies-arrayexpress

Gene expression of T47D-MTVL human breast cancer cells growing in normal conditions

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Project description:Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1X

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Gene expression by high-throughput sequencing of T47D-MTVL human breast cancer cells upon H1.4 knock-down and multiple H1 variants

Project description:Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1.4 (120sh) or multiple H1 variants (225sh)

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Gene expression of T47D-MTVL human breast cancer cells upon H1.4 knock-down or overexpression

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Gene expression of T47D-MTVL human breast cancer cells upon H1.4 knock-down or overexpression

Project description:Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1.4 (sh120) or multiple H1 variants (sh225), or overexpressing WT or K26A mutant HA-tagged H1.4. T47D-MTVL, breast cancer cell line carrying one stably integrated copy of luciferase reporter gene driven by the MMTV promoter, is stably infected with an inducible system for the expression of shRNAs.Cells stably express RedFP and KRAB repressor fused to Tet regulator.Upon Dox treatment, cells express RedFP and the cloned shRNA. Stable breast cancer-derived cell lines expressing an shRNA against one of each of the histone H1 isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: inducible shRNA against H1.4 or multiple H1 variants, and random shRNA-expression vector. Stable breast cancer-derived cell lines expressing the histone H1.4 isoform, WT or K26A, HA-tagged, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: overexpressing H1.4 WT or K26A mutant.

2014-05-16 | E-GEOD-55786 | biostudies-arrayexpress

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Project description:Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1X Stable breast cancer-derived cell lines expressing an shRNA against the histone H1X isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox, and serum-starved for the last 48h for synchronization in G1, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: inducible shRNA against H1X, and random shRNA-expression vector as a control.

2015-04-02 | E-GEOD-62766 | biostudies-arrayexpress

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