Project description:Chelonus formosanus is an important parasitic natural enemy of Lepidopteran insects and has great value in the biological control of insect pests. To screen the reference genes in the real-time fluorescence quantitative PCR (qRT-PCR) assay of Chelonus formosanus, six candidate reference genes were selected based on transcriptome data, including GAPDH, ACT, TU, TF, HSP, and RP. The expression stability of these six internal reference genes was determined by qRT-PCR in different tissues (head, chest, abdomen) and at different temperatures (11℃and 41℃), as well as with two insecticides (dinotefuran and chlorantraniliprole).
Project description:ArPHR2/4 acts as the core transcription factors in response to phosphorus starvation. They regulate the responsive signaling pathways, especially the phosphorus signaling cascade, thereby modulating the metabolic activities of downstream phosphate transporters. Overexpression of ArPHR2/4 in Anoectochilus roxburghii leaves markedly enhances the transcriptional activation of phosphorus-responsive genes. This finding provides valuable insights for exploring the phosphorus signaling pathway and associated metabolic processes.
