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RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

ABSTRACT: RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

PROVIDER: PRJNA997969 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25436800_1.fastq.gz	Fastqsanger.gz
	SRR25436800_2.fastq.gz	Fastqsanger.gz
	SRR25436801_1.fastq.gz	Fastqsanger.gz
	SRR25436801_2.fastq.gz	Fastqsanger.gz
	SRR25436802_1.fastq.gz	Fastqsanger.gz

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Human Gastric Cancer Cell line MKN45: Non-Target shRNA vs. SET/I2PP2A targeting shRNA

Project description:Transcriptional profiling of MKN45 cells comparing control stably expressing Non-Targeting shRNA cells with stably expressing SET/I2PP2A targeting shRNA cells. Goal was to determine the effects of SET knockdown on MKN45 gene expression.

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RNA transcriptome sequencing analysis of MKN45 cells transfected with tcons_00001221 shRNA or control shRNA

Project description:An RNA transcriptome sequencing analysis was performed in MKN45 cells that were transfected with tcons_00001221 shRNA or control shRNA.

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RNA transcriptome sequencing analysis of MKN45 cells transfected with tcons_00001221 shRNA or control shRNA

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Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

Project description:Gene expression profiles of in vitro selected highly metastatic MKN45-GFP sublines. The results were compared with MKN45-GFP control cell line to determine the metastasis associated genes. Four pairs compared experiment. Each pair was used MKN45-GFP cells as correlated control. Determining on the gene expression trends were by various metastatic ability of each subline.

2011-11-10 | E-GEOD-33570 | biostudies-arrayexpress

RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

Project description:Metastasis and recurrence are the major causes of poor outcome in gastric cancer (GC). We showed that ANKRD6 expression is associated with a poorer prognosis in 10 independent GC cohorts and with metastatic states and processes. Here, by transcriptomic profiling we have characterized the effect of ANKRD6 silencing in vitro and found that cells with ANKRD6 downregulation display an impairment in key metastatic processes such as the Epithelial-mesenchymal transition.

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Anti-proliferation activity of fucoidan on MKN45 gastric cancer cells and downregulation of phosphorylated ASK1, a cell-cycle-regulated cyclin-dependent kinase

Project description:Fucoidan is a high-molecular polysaccharide whose main constituent is sulfated fucose. Extensive studies have demonstrated numerous interesting biological activities for fucoidan. We specifically focused on the anti-proliferation activity of fucoidan and examined the underlying mechanism in MKN45 gastric cancer cells. BrdU assay revealed that fucoidan impeded the MKN45 cell cycle by approximately 50%, and clonogenic assay also showed that fucoidan inhibited cell proliferation. Preliminary examinations of fucoidan using LDH assay showed no immediate cytotoxic effects at 24-h exposure. However, longer time courses revealed inhibition of cell growth at 4 days in a dose-dependent manner. Microarray analysis in MKN45 cells treated with fucoidan identified genes that were upregulated and downregulated in response to fucoidan, including MAP3K5, or ASK1 (apoptosis signal-regulating kinase), which was upregulated by 1.38-fold. Western blot confirmed that fucoidan increased ASK1 protein levels, while reducing the levels of phosphorylated ASK1. Reduction of ASK1 by siRNA decreased proliferation of MKN45 cells. Our findings show that fucoidan may suppress cellular proliferation and DNA synthesis in MKN45 cells by suppressing the ASK1-p38 signaling pathway through reduction of phosphorylated ASK1 levels.

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Expression data from human stomach cell lines (MKN45 and MKN45P)

Project description:Analysis to find splicing variants that are differentially expressed in a highly metastatic stomach cancer cell line, MKN45P, versus its parental cell line, MKN45 Comparison between highly metastatic gastric cancer cell line MKN45P and its parental cell line MKN45

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Genes regulated by SET7/9 knockdown in gastric cancer cells

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