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ABSTRACT: Brain transcriptome analysis of sex reversal in Nile tilapia

PROVIDER: PRJNA999466 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25444359.fastq.gz	Fastqsanger.gz
	SRR25444360.fastq.gz	Fastqsanger.gz
	SRR25444361.fastq.gz	Fastqsanger.gz
	SRR25444362.fastq.gz	Fastqsanger.gz
	SRR25444363.fastq.gz	Fastqsanger.gz

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

Project description:In fish, the sex determining mechanisms can broadly be classified as genotypic (GSD), temperature-dependent (TSD), or genotypic plus temperature effects (GSD+TE). For the fish species with TSD or GSD+TE, extremely high or low temperature can affect its sex determination and differentiation. For long time, the underlying changes in DNA methylation that occur during high or low temperature induced sex reversal have not been fully clarified. In this study, we used Nile tilapia as a model to perform a genome-wide survey of differences in DNA methylation in female and male gonads between control and high temperature induced groups using methylated DNA immunoprecipitation (MeDIP). We identified the high temperature induction-related differentially methylated regions (DMRs), and performed functional enrichment analysis for genes exhibiting DMR. These identified differentially methylated genes were potentially involved in the connection between environmental temperature and sex reversal in Nile tilapia. In this study, four samples (control females, CF; control males, CM; induced females, IF; induced males, IM) were analyzed.

2015-08-27 | E-GEOD-72386 | biostudies-arrayexpress

Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

2015-08-27 | GSE72386 | GEO

Project description:Brain tissue reversal mRNA transcriptome analysis in Nile tilapia

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Project description:Nile tilapia brain transcriptome

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Oreochromis niloticus

Project description:Investigation of temperature-dependent sex reversal in Nile tilapia using ddRAD-seq

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Nile tilapia (Oreochromis niloticus) gene expression profiling in males and females at early embryonic development (2, 5 and 9 days post fertilization )

Project description:Commercial production of tilapia relies on monosex cultures of males, which so far proved difficult to maintain in large scale production facilities. Thus, a better understanding of the genetic architecture of the complex trait of sex determination in tilapia is needed.We aimed to detect genes that were differentially expressed by gender at early embryonic development. Artificial fertilization of O. niloticus females with either sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively. Pools of all-female and all-male embryos at 2, 5 and 9 days post fertilization were used for custom Agilent eArray. 56 pool samples of Nile tilapia full siblings groups (female or male) at day 2, 5 or 9 post fertilization were subjected to total RNA extraction from whole embryo tissues and hybridized to the custom Agilent array. Each sample was yielded from different cross of artificial fertilization: six dams X five sires. The resulting gender were known based on the sire, sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively.

2014-11-01 | E-GEOD-50974 | biostudies-arrayexpress

Characterization of elongation of the very long chain fatty acid protein 6 (elovl6) gene in male Oreochromis niloticus and its potential regulatory roles in lipid and glucose metabolism

Project description:Elongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of elovl6 and study regulatory mechanism of elovl6 on male Nile tilapia, Oreochromis niloticus. In the present study, the full-length cDNA of Nile tilapia elovl6 was 2255 bp, comprising a 5'-UTR of 193 bp, an ORF of 810 bp, and a 3'-UTR of 1252 bp, encoding 269 amino acids that possesses the characteristic features of Elovl proteins. The transcription expression of elovl6 transcripts showed differential expression in different tissues and fed with different lipid sources diet. Antisense RNA technology was used to knock-down elovl6 in Nile tilapia. Compared with the control treatments (transfected with ultrapure water (control) or the blank expression vector (negative control)), knockdown elovl6 altered hepatic morphology, Long chain fatty acid synthesis, fatty acid oxidation, lead to excess fat deposition in the liver, ultimately cause abnormal glucose/lipid metabolism in male Nile tilapia. Transcriptome analyses (AGPAT2 knock-down vs. negative control) revealed a total of 5877 differentially expressed genes (DEGs). Transcriptome analyses revealed DEGs were greatly involved in some signaling pathways, such as PPAR signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and insulin signaling pathway, which were related to lipid and glucose metabolism. We used qRT-PCR to verify the mRNA expression changes of 13 DEGs in related signaling pathways. Our results demonstrate that knock-down of elovl6 in tilapia blocks the synthesis of oleic acid and leads to abnormal glucose/lipid metabolism.

2025-11-01 | GSE248727 | GEO

Genome-wide microRNA screening in Nile tilapia reveals pervasive transcription of isomiRs, sex-biased arm switching and increasing complexity of expression throughout development

Project description:The elucidation of microRNA function and evolution depends on the identification and characterization of miRNA repertoire of strategic organisms, as the fast evolving cichlid fishes. Using RNA-seq and comparative genomics we carried out an in-depth report of miRNAs in Nile tilapia (Oreochromis niloticus). Our results enlarge vertebrate miRNAs collection and reveal a notable differential expression of miRNAs arms and isoforms influenced by sex and developmental life stage, providing a better picture of the evolutionary and spatiotemporal dynamics of miRNAs.

2019-03-01 | GSE102878 | GEO

Project description:Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia

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Epigenetic mapping of the somatotropic axis reveals differential DNA hydroxymethylation marks with potential implication in growth

Project description:We report an association of DNA hydroxymethylation profiling at single nucleotide resolution with gene expression in the fast muscle of Nile tilapia.

2021-07-27 | GSE158910 | GEO

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