Ontology highlight
ABSTRACT:
PROVIDER: PRJNA999588 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR25445371_1.fastq.gz | Fastqsanger.gz | |||
| SRR25445371_2.fastq.gz | Fastqsanger.gz | |||
| SRR25445372_1.fastq.gz | Fastqsanger.gz | |||
| SRR25445372_2.fastq.gz | Fastqsanger.gz | |||
| SRR25445373_1.fastq.gz | Fastqsanger.gz |
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Project description:KMD is genetically engenered to be highly resistant to lepidopteran pests through expressing a synthetic cry1Ab gene and its parent non-transgenic rice is Xiushui 11.The developmental duration of BPH feeding on KMD2 was significantly delayed. And moreover, the fecundity of BPH was significantly lower when fed on Bt rice than on the non-Bt parental plants.To investigate unintended effects in KMD2 that causes changes in BPH performance, we performed microarray (GeneChip) analysis to compare the gene expression profiles between Bt rice and non-transgenic parental plants in response to BPH infestation. We used microarrays to detect Bt-independent variation, which might render Bt rice more defensive or less nutritious to BPH. For BPH treatment, 10 second-instar nymphs were infested onto each 30-day-old seedling. After 72 h, the BPH nymphs were carefully removed and rice shoots of both BPH-infested and non-infested plants were sampled for microarray analysis. There were four treatments: Xiushui 11-non infested, Xiushui 11-BPH infested, KMD2-non infested, KMD2-BPH infested, three biological replications.
2015-11-06 | E-GEOD-74745 | biostudies-arrayexpress
Project description:The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is a major insect pest of cultivated rice in temperate Asia. GRH2-near-isogenic line (NIL) TGRH11, GRH4-NIL (TGRH16) and GRH2/GRH4-pyramided line (PYL) TGRH29 were developed by introducing the GRH2 and GRH4 from indica rice (DV85). We identified GRH-inducible genes in respective rice lines. Furthermore, we compared the gene expression levels between NILs/PYL and control plants (T65). The gene expression changes in respective rice lines were detected by comparison between GRH-infested and pre-infested plants. Seedlings at the second-leaf stage were infested with 10 to 15 first- or second-instar nymphs in test tubes and shoots were collected at 30 h after GRH infestation. Detached-leaf blades at heading stage were infested with 10 to 15 first- or second-instar nymphs in test tubes and samples were collected at 30 h after GRH infestation. For each treatment, two biological replicates were performed.
2016-04-02 | E-GEOD-46409 | biostudies-arrayexpress
Project description:Lipid metabolism and antioxidant system contributed to salt salinity tolerance induced by Na+ accumulation in halophytic grass seashore paspalum
2023-05-23 | GSE233155 | GEO
Project description:<p>1. The ecological niche of aphids is largely defined by the phloem sap of plants, which they consume. Previous studies revealed that aphids may change the phloem sap metabolome and that a previous aphid infestation may benefit them. As aphids reproduce parthenogenetically, the performance and preference of aphids of the same monoclonal aphid lineage can be compared between previously uninfested and previously aphid-infested plants.</p><p>2. Wheat (<em>Triticum aestivum</em>) leaves were infested by clonal lineages of <em>Sitobion avenae</em> aphids for 9–10 days. Leaf phloem exudates were analysed using amino acid profiling and metabolic fingerprinting, capturing mainly specialised metabolites. It was investigated whether aphid colony sizes differ between leaves that were previously uninfested or infested by aphids of a different clonal lineage or by aphids of the same clonal lineage and whether aphids show a preference for any of these leaves.</p><p>3. The relative concentrations of metabolites in the phloem exudates were not affected by aphid infestation. Aphid colonies were partly larger on previously aphid-infested leaves than on control leaves. However, this effect was transient and finally colonies had similar sizes. Given a choice between previously aphid-infested and uninfested leaves, <em>S. avenae</em> nymphs showed no preferences.</p><p>4. The findings that positive effects of previous aphid infestation on aphid performance are transient and that the performance but not the preference of the aphids was affected by previous aphid infestation highlight the temporal dynamics and complexity of aphid–plant interactions. Niche alteration processes may be partly involved in these interactions.</p>
2025-02-25 | MTBLS7626 | MetaboLights
Project description:Ixodes species ticks are competent vectors of tick-borne viruses including tick-borne encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate and enhance viral infection. This likely occurs by saliva-mediated modulation of host responses into patterns favorable for viral infection and dissemination. Because of the rapid kinetics of tick-borne viral transmission, this modulation must occur as early as tick attachment and initiation of feeding. In this study, the gene expression profile of cutaneous bite-site lesions created by uninfected ticks were analyzed at 1, 3, 6, and 12 hours after Ixodes scapularis nymphal tick attachment to discover host pathways or responses potentially important in tick-borne viral establishment. Four milimeter ear biopsies from BALB/cJ mice infested with Ixodes scapularis nymphs were assayed using Affymetrix genechip 430A 2.0 arrays at 1, 3, 6, and 12 hours after infestation during a primary exposure. 3 mice were measured at each time point. Controls were 3 similarly housed but tick-free mice.
2012-12-21 | E-GEOD-39100 | biostudies-arrayexpress
Project description:BACKGROUND: The tomato psyllid, Bactericera cockerelli Šulc (Hemiptera: Triozidae), is a pest of solanaceous crops such as tomato (Solanum lycopersicum L.) in the U.S. and vectors the disease-causing pathogen ‘Candidatus Liberibacter solanacearum’. Currently, the only effective strategies for controlling the diseases associated with this pathogen involve regular pesticide applications to manage psyllid population density. However, such practices are unsustainable and will eventually lead to widespread pesticide resistance in psyllids. Therefore, new control strategies must be developed to increase host-plant resistance to insect vectors. For example, expression of constitutive and inducible plant defenses can be improved through selection. Currently, it is still unknown whether psyllid infestation has any lasting consequences on tomato plant defense or tomato plant gene expression in general. RESULTS: To characterize the genes putatively involved in tomato defense against psyllid infestation, RNA was extracted from psyllid-infested and uninfested tomato leaves (Moneymaker) three weeks post-infestation. Transcriptome analysis identified 362 differentially expressed genes. These differentially expressed genes were primarily associated with defense responses to abiotic/biotic stress, transcription/translation, cellular signaling/transport, and photosynthesis. These gene expression changes suggested that tomato plants underwent a reduction in plant growth/health in exchange for improved defense against stress that was observable three weeks after psyllid infestation. Consistent with these observations, tomato plant growth experiments determined that the plants were shorter three weeks after psyllid infestation. Furthermore, psyllid nymphs had lower survival rates on tomato plants that had been previously psyllid infested. CONCLUSION: These results suggested that psyllid infestation has lasting consequences for tomato gene expression, defense, and growth.
2021-01-30 | GSE165807 | GEO