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ProteomicsIn-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensi...
ORGANISM(S): Dermatophagoides Pteronyssinus Saccharomyces Cerevisiae 
2024-01-26 | PXD045568 | panorama
UnknownDevelopment of an open source laboratory information management system for 2-D gel electrophoresis-based proteomics workflow.
Not available
S-EPMC1599757 | biostudies-literature
UnknownUsing differential solubilization and 2-D gel electrophoresis to visualize increased numbers of proteins in the human cortex and caudate nucleus and putamen.
Not available
S-EPMC3056166 | biostudies-literature
UnknownSerum and tissue proteomic signatures of patients with hepatocellular carcinoma using 2‑D gel electrophoresis.
Not available
S-EPMC6625405 | biostudies-literature
ProteomicsBlue native gel electrophoresis of Syntrophus aciditrophicus proteome
Blue native gel electrophoresis of Syntrophus aciditrophicus proteome
ORGANISM(S): Syntrophus Aciditrophicus Sb 
2019-04-29 | PXD011101 | JPOST Repository
ProteomicsPhos-tag SDS-PAGE followed by LC-MS/MS analysis of in vitro phosphorylated Nab2, Foxk1, and HURP
Purified GST-fused Nab2, Foxk1, and HURP were incubated with ATP in the presence or absence of His-tagged active ERK in vitro, separated by Phos-tag SDS-PAGE, and visualized by CBB staining. The most shifted regions of protein bands on the Phos-tag-containing gels were excised, digested with trypsin...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028837 | JPOST Repository
ProteomicsCharacterization of the Zein Protein Composition in Maize Flour Extracts
Highly homogenous zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-ge...
ORGANISM(S): Zea mays (Maize) 
2019-06-11 | PXD012849 | Pride
Zein proteins Peptide mass fingerprinting Data base search Maize Maldi-ms Custom-built sequence data base Flour 2d gel electrophoresis 
TranscriptomicsSmall RNAs in rice seed
Small RNAs are common and effective modulators of gene expression in eukaryotic organisms. To characterize the small RNAs expressed during rice seed development, massively parallel signature sequencing (MPSS) was performed, resulting in the obtainment of 22-nt sequence signatures. Through integrativ...
ORGANISM(S): Oryza sativa 
2009-02-02 | E-GEOD-11974 | biostudies-arrayexpress
TranscriptomicsKaryotyping of maltose-fermenting Saccharomyces yeasts with differing maltotriose utilization profiles
Aims: We performed an analysis of maltotriose utilization by 52 Saccharomyces yeast strains able to ferment maltose efficiently and correlated the observed phenotypes with differences in the copy number of genes possibly involved in maltotriose utilization by yeast cells. Methods and Results: The an...
ORGANISM(S): Saccharomyces cerevisiae 
2010-07-22 | E-GEOD-23055 | biostudies-arrayexpress
UnknownOptimizing human synovial fluid preparation for two-dimensional gel electrophoresis.
Not available
S-EPMC3206423 | biostudies-literature
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