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ProteomicsXL-MS of 5S RNP preribosomal complexes
The 5S RNP is assembled from its three components (5S rRNA, Rpl5/uL18, and Rpl11/uL5) before being incorporated into the pre-60S subunit. However, when ribosome synthesis is disturbed, a free 5S RNP can enter the MDM2–p53 pathway to regulate cell cycle and apoptotic signaling. Here we reconstitute a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-06-13 | PXD040087 | Pride
5s rnp Cryo-em Mdm2 
ProteomicsRibosome protein composition in wt and 23S+5S rRNA ribosomes
The 120-nt long 5S rRNA, is an indispensable component of cytoplasmic ribosomes in all living organisms. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether maintaining 5S rRNA ...
ORGANISM(S): Escherichia coli 
2020-06-18 | PXD019058 | Pride
Mass-spectrometry Ribosome Proteins 
ProteomicsBacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism
Here, we present the crystal structure of the catalytic domain of M5 bound to two magnesium ions, which reveals the role of each catalytic site acidic residue in metal binding. We further use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to investigate the possibility of structur...
ORGANISM(S): Geobacillus stearothermophilus 
2021-02-10 | PXD019202 | Pride
Rnase Native-ms Hdx-ms 
OtherSaturation mutagenesis of the human 5S rRNA gene defines expression- and ribosome-incorporation-defective variants
The transcribed 5S rRNA gene of the human 5S rDNA array was saturation-mutagenised in an expression plasmid and transfected into HEK293T cells. Cells were fractionated on sucrose gradients and 5S rRNA sequenced from the input (total) RNA and the assembled 60S large ribosomal subunit, alongside the i...
ORGANISM(S): Homo sapiens 
2026-09-02 | GSE339543 | GEO
TranscriptomicsUBF Is An Epigenetic Factor Required For Ribosomal RNA Gene Activity And Preinitiation Complex Assembly But Not For Nucleolar Body Formation
Upstream Binding Factor (UBF) is a unique multi-HMGB-box protein first identified as a co-factor in RNA polymerase I (RPI/PolI) transcription. However, its poor DNA sequence selectivity and its ability to generate nucleosome-like nucleoprotein complexes suggest a more generalized role in chromatin s...
ORGANISM(S): Mus musculus 
2014-08-19 | E-GEOD-55450 | biostudies-arrayexpress
UnknownEukaryotic 5S rRNA biogenesis.
Not available
S-EPMC3278907 | biostudies-literature
UnknownThe participation of 5S rRNA in the co-translational formation of a eukaryotic 5S ribonucleoprotein complex.
Not available
S-EPMC55736 | biostudies-literature
UnknownDicistronic tRNA-5S rRNA genes in Yarrowia lipolytica: an alternative TFIIIA-independent way for expression of 5S rRNA genes.
Not available
S-EPMC2566860 | biostudies-literature
GenomicsSaturation mutagenesis of the human 5S rRNA gene defines expression- and ribosome-incorporation-defective variants
Saturation mutagenesis of the human 5S rRNA gene defines expression- and ribosome-incorporation-defective variants
PRJNA1499145 | ENA
OtherChaperoning 5S RNA assembly by structural probing
In eukaryotes, three of the four ribosomal RNAs (rRNAs), the 5.8S, 18S and 25S/28S rRNAs, are processed from a single pre-rRNA transcript and assembled into ribosomes. The fourth rRNA, the 5S rRNA, is transcribed by RNA polymerase III and is assembled into the 5S ribonucleoprotein particle (5S RNP),...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2015-07-20 | GSE68431 | GEO
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