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In order to better understand bacterial aggregates mechanisms we used RNA seq and a specific setup of E. coli K12 strains expressing either the natural self-recognizing adhesin Ag43 or nanobodies based synthetic adhesins. We studied the changes in gene expression between aggregates and planktonic ce...
ORGANISM(S): Escherichia coli O17 str. K12a 
Colibactin-driven colon cancer requires adhesin-mediated epithelial binding
To obtain RgpA free of adhesin requires a means of separating the RgpA catalytic domain from the adhesins domains or adaptation of a method to prevent RgpA complex association.In this study, recombinant suicide plasmids were designed for use in homologous recombination with the rgpA locus so as to p...
ORGANISM(S): Porphyromonas gingivalis W50 
2023-06-02 | PXD036859 | Pride
Various bacteria are suggested to contribute to colorectal cancer (CRC) development, including pks+ E. coli, which produces the genotoxin colibactin that induces characteristic mutational signatures in host epithelial cells. However, it remains unclear how the highly unstable colibactin molecule is ...
ORGANISM(S): Mus musculus 
2024-08-19 | GSE241851 | GEO
Transcriptomic analysis of E. coli aggregates reveals both a general and an adhesin-specific responses
The adhesin RadD enhances Fusobacterium nucleatum tumor colonization and colorectal carcinogenesis
The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the...
ORGANISM(S): Homo sapiens (Human) Brachyspira pilosicoli P43/6/78 
2024-10-18 | PXD056942 | Pride
Eukaryotic cell growth is coordinated in response to nutrient availability, growth factors, and environmental stimuli, enabling cell–cell interactions that promote survival. The rapamycin-sensitive Tor1 protein kinase, which is conserved from yeasts to humans, participates in a signaling pathway cen...
ORGANISM(S): Candida albicans 
2009-02-21 | GSE13176 | GEO
The experimental project studied a MIDAS adhesin minus mutant of predatory bacterium B. bacteriovorus.The predatory bacterium normally invades and lives inside E.coli bacteria, rounding them up to form a two-bacterial structure, called a bdelloplast, and killing the E.coli from the inside. However ...
ORGANISM(S): Bdellovibrio bacteriovorus HD100 
2024-03-18 | PXD050423 | Pride
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