Intracellular proteome aggregation is a ubiquitous disease hallmark with its composition associated with pathogenicity. Herein, we report on a cell-permeable photosensitizer (P8, Rose Bengal derivative) for selective photo-induced proximity labeling, crosslinking and identification of cellular aggre...
MDK is a heparin-binding growth factor and found to be the Aβ associated protein. Through aggregated proteome, we aim to identify DE proteins more associated with plaques by MDK knockout.
Gmdnj1-knockout mutants had diminished growth in normal conditions, and when under heat stress, exhibited more severe browning, reduced chlorophyll contents, higher reactive oxygen species (ROS) contents, and higher induction of heat stress-responsive transcription factors and ROS-scavenging enzyme-...
This chemical proteomics method (AggLink method) can profile the composition of amorphous aggregated proteome in stressed cells and reveal its heterogeneity.
Analyzing and comparing proteome profiling between human control and AD cases will be beneficial to understand the molecular changes of insoluble fractions of AD cases.
We extracted DIP from placanta tissue of human which were diagnose preeclampsia or normal. The samples of DIPs were digested by FAST and the peptides were used DIA to analysis.
